Ann Clin Microbiol.  2014 Jun;17(2):50-57. 10.5145/ACM.2014.17.2.50.

Characterization of Salmonella spp. Clinical Isolates in Gyeongsangbuk-do Province, 2012 to 2013

• Kweon OG
• Kim JS
• Kim GO
• Lee CI
• Jeong KH
• Kim J

• KMID: 1971094
• DOI: http://doi.org/10.5145/ACM.2014.17.2.50

Abstract

BACKGROUND
Extended-spectrum cephalosporins and fluoroquinolones are important antimicrobials for treating invasive salmonellosis, and emerging resistance to these antimicrobials is of paramount concern.
METHODS
A total of 30 Salmonella spp. clinical isolates recovered in Gyeongsangbuk-do from 2012 to 2013 were characterized using antibiotic resistance profiles and pulsed-field gel electrophoresis (PFGE).
RESULTS
A high prevalence of multidrug-resistant isolates, mainly showing an ampicillin, nalidixic acid, chloramphenicol resistance pattern, was observed. Four extended-spectrum beta-lactamase (ESBL)-producing isolates (3 CTX-M-15 isolates and 1 CTX-M-27 isolate) were found. The bla(CTX-M-27) gene was carried by an IncF conjugative plasmid in the S. Infantis isolate. The bla(CTX-M-15) gene were carried by an IncF (2 isolates) or IncHI2 (1 isolate) conjugative plasmid in S. Enteritidis. In addition, a single mutation of GyrA, Ser83Thr (1 isolates), Asp87Tyr (9 isolates), Asp87Gly (4 isolates), and Asp87Leu (3 isolates), was detected in nalidixic acid-resistant Salmonella spp. isolates. XbaI PFGE analysis of all isolates revealed more than 19 different pulsotypes. The most common S. Enteritidis PFGE pattern (SEGX01.003) was associated with a larger number of cases of invasive salmonellosis than all other patterns.
CONCLUSION
The information from our study can assist in source attribution, outbreak investigations, and tailoring of interventions to maximize disease prevention.

Keyword

Drug resistance; Extended-spectrum beta-lactamase; Nalidixic acid; Pulsed-Field Gel Electrophoresis; Salmonella

MeSH Terms

Ampicillin
beta-Lactamases
Cephalosporins
Chloramphenicol Resistance
Drug Resistance
Drug Resistance, Microbial
Electrophoresis, Gel, Pulsed-Field
Fluoroquinolones
Gyeongsangbuk-do
Nalidixic Acid
Plasmids
Prevalence
Salmonella Infections
Salmonella*
Ampicillin
Cephalosporins
Fluoroquinolones
Nalidixic Acid
beta-Lactamases

Figure

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  Fig. 1. Clustering of XbaI digested PFGE patterns for Salmonella spp..

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