Ewha Med J.  1979 Dec;2(4):195-199. 10.12771/emj.1979.2.4.195.

Effect of Thiamine Deficiency on Drug Hydroxylation and Level of Cytochrome P-450 in the Rats

• Kim BH
• Hong YS
• Sung NE

• KMID: 1969656
• DOI: http://doi.org/10.12771/emj.1979.2.4.195

Abstract

Our studies are concerned with cytochrome P-450 content and 2-acetylaminofluorene N-and ring-hydroxylation by thiamine deficient rat liver microsomes. Incubation medium contained 100mM HEPES buffer pH 7.8, 2mM NADPH, 100mM KF, 100nM [14C]-AAF and microsomal protein. After 30min, incubation at 37degrees C, ring and N-hydroxy-9-14C-AAF formations are assayed by radioactivity measurements after paper chromatography separation. The concentration of cytochrome P-450 is elevated in microsomes from rats fed the deficient diet. It appears that the thiamine deficient rat liver is capable of producing effects on the drug hydroxylation enzymes.

MeSH Terms

2-Acetylaminofluorene
Animals
Chromatography, Paper
Cytochrome P-450 Enzyme System*
Cytochromes*
Diet
HEPES
Hydrogen-Ion Concentration
Hydroxylation*
Liver
Microsomes
Microsomes, Liver
NADP
Radioactivity
Rats*
Thiamine Deficiency*
Thiamine*
2-Acetylaminofluorene
Cytochrome P-450 Enzyme System
Cytochromes
HEPES
NADP
Thiamine