J Korean Assoc Maxillofac Plast Reconstr Surg.  1997 Aug;19(3):265-286.

Experimental Study On The Bone Induction Capacity Of The Porcine Bone Matrix-Derived Bone Morphogenetic Protein

• Park YW
• Lee JH
• Kim SK

Abstract

Bone morphogenetic proteins(BMPs) are a group of transforming growth factor beta(TGF-beta)-related factors and multifunctional proteins, especially the only known biologic factors capable of inducing endochondral bone formation at an extraskeletal site. This study was performed to investigate the effect of the partially purified porcine BMP(pBMP) at an ectopic site. PBMP was partially purified from porcine bone matrix and its activity was monitored by an in vivo bioassay. The purification method utilized extraction of the bone-including actively with 4M guanidine, followed by chromatography on heparin-Sepharose. Active fractions were reconstituted with inactive insoluble collagenous bone matrix from rats, acid soluble type I collagen from rat tail and chondroitin-6-sulfate sodium salt and implanted into the pectroralis muscle as control. The rats were sacrificed at the day of 1st, 3rd 5th. 7th, 11th, 14th and 21st after implantation and examined histologically, radiologically and biochemically. And alkaline phosphatase activity and calcium content were used as indices of bone formation. The results were as follows ; 1. Active fractions were localized in a zone between 31 and 40 KDa on SDS-PAGE 2. The implanted 3.0mg of the partially purified pBMP induced cartilage and bone in the muscle tissue of rats through an endochondral ossification process. 3. Inactive insoluble bone matrix, type I collagen and chondroitin-6-sulfate have functioned as carriers for pBMP, but revealed some foreign body reactions. 4. Soft X-ray didn't reveal significant change between the experimental and the control group. 5. The alkaline phosphatase activities in the experimental group of 5th. 7th, 11th. 14th and 21st were increased significantly compared with control(p<0.01) with the peak in the group of 11th day. 6. With time, the calcium content of the experimental group increased. And the calcium cintents in the experimental group of 11th, 14th, and 21 were increased significantly compared with control(p&0.01).

MeSH Terms

Alkaline Phosphatase
Animals
Biological Assay
Biological Factors
Bone Matrix
Bone Morphogenetic Proteins*
Calcium
Cartilage
Chromatography
Collagen
Collagen Type I
Electrophoresis, Polyacrylamide Gel
Foreign Bodies
Guanidine
Osteogenesis
Rats
Sodium
Tail
Transforming Growth Factors
Alkaline Phosphatase
Biological Factors
Bone Morphogenetic Proteins
Calcium
Collagen
Collagen Type I
Guanidine
Sodium
Transforming Growth Factors