J Korean Rheum Assoc.  2001 Sep;8(3):160-168.

The Effects of Interleukin-17 on Production of Vascular Endothelial Growth Factor in Cultured Rheumatoid Arthritis Synoviocytes

  • 1Department of Internal Medicine, College of Medicine, Pusan National University, Pusan, Korea. ksimd@hyowon.pusan.ac.kr
  • 2Department of Orthopedics, College of Medicine, Pusan National University, Pusan, Korea.
  • 3Pusan National University Hospital Institute, Pusan, Korea.


To investigate the the effects of interleukin-17 (IL-17)on the production of vascular endothelial growth factor (VEGF)from cultured rheumatoid arthritis synoviocytes.
Fibroblast-like synovial cells(FLS)were prepared from the synovial tissues of rheumatoid arthritis patients and cultured in the presence of IL-17, IL-17 with or without transforming growth factor-beta(TGF-beta),tumor necrosis factor-alpha(TNF-alpha)and interleukin-1 beta(IL-1 beta).VEGF levels were determined in the culture supernatants by sandwitch ELISA.
Stimulation of FLS by serial concentration of IL-17,TGF-beta,TNF-alpha,IL-1 beta increased the production of VEGF by 2.1-2.7,2.2-3.0,2.0-2.9,2.3-3.1 fold over the constitutive levels of unstimulated FLS.Stimulation of FLS by IL- 17 with TGF-beta or TNF-alpha or IL-1 beta also increased the production of VEGF accord-ing to culture periods by 1.6-1.8,1.1-1.9,1.5-1.7 fold over the levels stimulated with TGF-beta or TNF-alpha or IL-1 beta,respectively.This results indicated that IL-17 increased the effect of TGF-beta,TNF-alpha,IL-1 beta on FLS,leading synergistic enhancement of VEGF production.
IL-17 may be involved in the neovascularization in rheumatoid synovitis by enhancing the production of VEGF.


Interleukin-17; Vascular endothelial growth factor; Rheumatoid arthritis synoviocytes
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