J Korean Rheum Assoc.  2004 Mar;11(1):1-6.

The Effects of Interleukin-17 on Production of Matrix Metalloproteinase-3 in Cultured Rheumatoid Arthritis Synoviocytes

Affiliations
  • 1Medical Research Institute, Pusan National University Hospital, Busan, Korea. ksimd@pusan.ac.kr
  • 2Department of Internal Medicine, College of Medicine, Pusan National University, Busan, Korea.

Abstract


OBJECTIVE
To investigate the effect of interleukin-17 (IL-17) on the production of matrix metalloproteinase-3 (MMP-3) and the expression of MMP-3 mRNA in rheumatoid arthritis synoviocytes.
METHODS
Fibroblast-like synovial cells (FLS) were prepared from the synovial tissues of rheumatoid arthritis patients and cultured in the presence of various concentration of IL-17. The production of MMP-3 and expression of MMP-3 mRNA were determined by sandwitch ELISA and real-time quantitative RT-PCR.
RESULTS
In ELISA, stimulation of FLS by IL-17 with 5, 10, 20, 40 ng/mL concentrations increased the production of MMP-3 by 2.7, 2.9, 3.1, 3.6 fold after 24 hours and 9.9, 10, 11, 12 fold after 48 hours over the constitutive levels of unstimulated FLS. In real-time quantitative RT-PCR, stimulation of FLS by IL-17 with 5, 10, 20, 40 ng/mL concentrations increased the MMP-3/beta-actin mRNA ratio by 3.2, 5.7, 9.9, 30.5 fold after 24 hours over the constitutive levels of unstimulated FLS.
CONCLUSIONS
IL-17 may be involved in the joint destruction in rheumatoid arthritis by enhancing the production of MMP-3 from rheumatoid arthritis synoviocytes.

Keyword

Rheumatoid arthritis synoviocytes; MMP-3; IL-17
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