Korean J Anat.
2006 Apr;39(2):103-110.
Inhibition of p65 Nuclear Translocation by KIOM-79
- Affiliations
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- 1Department of Herbal Pharmaceutical Development, Korea Institute of Oriental Medicine, Korea.
- 2Department of Anatomy, College of Medicine, Chosun University, Korea. iyjang@chosun.ac.kr
- 3Department of Pharmacology, College of Medicine, Chosun University, Korea.
- 4Department of Urology, College of Medicine, Chosun University, Korea.
- 5Department of Anatomy, College of Medicine, Seonam University, Korea.
- 6Department of Anatomy, College of Medicine, Kangwon National University, Korea.
Abstract
- We demonstrate that KIOM-79, combined extracts isolated from Magnolia officinalis, Pueraria lobata, Glycyrrhiza uralensis, and Euphorbia pekinensis, inhibits LPS-induced expression of iNOS gene in RAW 264.7 cells. Treatment of RAW 264.7 cells with KIOM-79 inhibited LPS-stimulated nitric oxide production in a doserelated manner. Immunohisto-chemical staining of iNOS and RT-PCR analysis showed that the decrease of NO was due to the inhibition of iNOS gene expression. Immunostaining of p65 and EMSA showed that KIOM-79 inhibited NF-kappa/Rel nuclear translocation and DNA binding, respectively. Collectively, this series of experiments indicates that KIOM inhibits iNOS gene expression by blocking NF-kappa/Rel. Due to the critical role that NO release plays in mediating inflammatory responses, the inhibitory effects of KIOM-79 on iNOS suggest that KIOM-79 may represent a useful anti-inflammatory agent.
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