Korean J Anesthesiol.  2003 Feb;44(2):255-264. 10.4097/kjae.2003.44.2.255.

Spinal and Supraspinal Changes in Vitamin D3 Up-regulated Protein 1 (VDUP1) Gene Expression after Spinal Cord Injury

• Shim YH
• Lee YH
• Lee WH
• Yoon SH
• Shin YS

• KMID: 1936001
• DOI: http://doi.org/10.4097/kjae.2003.44.2.255

Abstract

BACKGROUND: Vitamin D3 up-regulated protein 1 (VDUP1) gene is known to be a novel member of the early response genes and an oxidative stress mediator. This study was designed to elucidate VDUP1 expression and its involvement in central sensitization after spinal cord injury (SCI).
METHODS
Contusion injury was produced at spinal segment T10 (20 mm drop, 10 g rod) in adult male Sprague-Dawley rats (250 300 g). Withdrawal responses were measured using von Frey filaments and acetone on the 1st, 3rd and 7th days after SCI. The expressions of VDUP1 gene in the brain and in the cervical and lumbar spine were examined by immunohistochemistry on the 1st, 3rd and 7th day after SCI.
RESULTS
VDUP1 gene was detected in a few oligodendrocytes in the spinal cord and in the brain of control rats. VDUP1 gene expression increased in most of the neurons and ependymal cells in the central canal of the injured (lumbar) spinal cord 1 day after SCI. This expression gradually decreased in majority of cells from day 1 to day 7 after SCI. VDUP1 gene expression was also observed to be increased 1 day after SCI, and gradually to decrease from 1 day to 7 days after SCI. The neurons in the intact (cervical) spinal cord VDUP1 gene expression increases maximally 3 days after SCI in the cerebral cortex and the thalamus. Neuropathic pain behavior was triggered by the plantar surface of the fore foot after SCI.
CONCLUSIONS
These results show that the VDUP1 gene may be an early modulator of transneuronal stress response after SCI, and to be related to the central sensitization of neuropathic pain behavior after SCI.

Keyword

Central sensitization; gene; neuropathic pain; spinal cord injury; vitamine D3 up-regulated protein

MeSH Terms

Acetone
Adult
Animals
Brain
Central Nervous System Sensitization
Cerebral Cortex
Cholecalciferol*
Contusions
Foot
Gene Expression*
Humans
Immunohistochemistry
Male
Neuralgia
Neurons
Oligodendroglia
Oxidative Stress
Rats
Rats, Sprague-Dawley
Spinal Cord Injuries*
Spinal Cord*
Spine
Thalamus
Vitamins*
Acetone
Cholecalciferol
Vitamins