Korean J Obstet Gynecol.  1997 Nov;40(11):2568-2572.

Study on the Detection of Chromosome X, Y Using in situ PCR

• Kim YJ

Abstract

Untill now, classical karyotyping remains the most reliable and conclusive method, but sometimes very rapid and sensitive method for the detection of chromosomal abnormality is needed. The primed in situ labelling(PRINS) is very rapid and sensitive method for the detection of chromosomal aneuploidy and X-linked disorder instead of fluorescen in situ hybridization. The purpose of this study was to prepent the application of PRINS protocol to the rapid detection of chromosomes X and Y in both metaphases and interphase nuclei. The PRINS reaction was done in 10 amniocytes. We compared the PRINS result with conventional cytogenetic karyotyping. The result was the same with cytogenetic karyotyping. In the future, PRINS will become very useful and sensitive method for the chromosomal aneuploidy detection and X-linked disorder diagnosis.

Keyword

In situ PCR; PRINS; Chromosome; Prenatal diagnosis

MeSH Terms

Aneuploidy
Chromosome Aberrations
Cytogenetics
Diagnosis
In Situ Hybridization
Interphase
Karyotyping
Metaphase
Polymerase Chain Reaction*
Prenatal Diagnosis
Primed In Situ Labeling