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Poorly-Controlled Type 1 Diabetes Mellitus Impairs LH-LHCGR Signaling in the Ovaries and Decreases Female Fertility in Mice

Lee J, Lee HC, Kim SY, Cho GJ, Woodruff T

PURPOSE: The aim of this study was to investigate how type I diabetes mellitus (T1D) affects the folliculogenesis and oocyte development, fertilization, and embryo development. MATERIALS AND METHODS: A comparative animal...
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Various macromolecules in in vitro growth medium influence growth, maturation, and parthenogenetic development of pig oocytes derived from small antral follicles

Lee H, Lee Y, Lee J, Lee GS, Lee ST, Lee E

This study was performed to examine the effects of various macromolecules in in vitro growth (IVG) media on the growth, maturation, and parthenogenesis (PA) of pig oocytes derived from small...
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Anaplastic Large Cell Lymphoma with Massive Eosinophilia and Complex Karyotype Initially Misdiagnosed as Chronic Eosinophilic Leukemia

So MK, Park S, Cho MS, Mun YC, Huh J

We report a patient with massive eosinophilia and a complex karyotype that was initially misdiagnosed as chronic eosinophilic leukemia (CEL), but later diagnosed as anaplastic large cell lymphoma (ALCL) masked...
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Clinicopathological Characteristics of Hyperdiploidy with High-Risk Cytogenetics in Multiple Myeloma

Yang N, Mun YC, Seong CM, Huh HJ, Huh J

In multiple myeloma (MM), hyperdiploidy (HD) is known to impart longer overall survival. However, it is unclear whether coexistent HD ameliorates the adverse effects of known high-risk cytogenetics in MM...
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The association of follicular fluid volume with human oolemma stretchability during intracytoplasmic sperm injection

Inoue T, Yamashita , Tsujimoto , Yamamoto S, Taguchi S, Hirao K, Uemura M, Ikawa K, Miyazaki K

OBJECTIVE: Oocyte degeneration often occurs after intracytoplasmic sperm injection (ICSI), and the risk factor is low-quality oocytes. The follicular fluid (FF) provides a crucial microenvironment for oocyte development. We investigated...
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Frequency and Distinct Characteristics of Acute Myeloid Leukemia Lacking HLA-DR and CD34 Expression: Features Intermediate between Typical Acute Myeloid Leukemia and Acute Promyelocytic Leukemia

Lee HY, Cho YU, You E, Jang S, Seo EJ, Park CJ

BACKGROUND: The objective of this study was to investigate the frequency and characteristics of HLA-DR⁻/CD34⁻ acute myeloid leukemia (AML) also known as acute promyelocytic leukemia (APL)-like AML. METHODS: This study included...
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The effects of different types of media on in vitro maturation outcomes of human germinal vesicle oocytes retrieved in intracytoplasmic sperm injection cycles

Fesahat F, Dehghani Firouzabadi R, Faramarzi , Khalili MA

OBJECTIVE: Optimizing in vitro maturation (IVM) media to achieve better outcomes has been a matter of interest in recent years. The aim of this prospective clinical trial was to investigate...
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Dysregulation of Telomere Lengths and Telomerase Activity in Myelodysplastic Syndrome

Park HS, Choi J, See CJ, Kim JA, Park SN, Im K, Kim SM, Lee DS, Hwang SM

BACKGROUND: Telomere shortening is thought to be involved in the pathophysiology of myeloid malignancies, but telomere lengths (TL) during interphase and metaphase in hematopoietic malignancies have not been analyzed. We...
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Effects of sperm insemination on the final meiotic maturation of mouse oocytes arrested at metaphase I after in vitro maturation

Yoon J, Juhn KM, Yoon SH, Ko Y, Lim JH

OBJECTIVE: The aims of this study were to investigate whether fertilization could induce the resumption of meiosis in mouse oocytes arrested at metaphase I (MI) after in vitro maturation (IVM),...
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The p90 ribosomal S6 kinase 2 specifically affects mitotic progression by regulating the basal level, distribution and stability of mitotic spindles

Park YY, Nam HJ, Do M, Lee JH

RSK2, also known as RPS6KA3 (ribosomal protein S6 kinase, 90 kDa, polypeptide 3), is a downstream kinase of the mitogen-activated protein kinase (MAPK) pathway, which is important in regulating survival, transcription,...
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A retrospective analysis of cytogenetic alterations in patients with newly diagnosed multiple myeloma: a single center study in Korea

Li S, Lim HH, Woo KS, Kim SH, Han JY

BACKGROUND: The accurate identification of cytogenetic abnormalities in multiple myeloma (MM) has become more important over recent years for the development of new diagnostic and prognostic markers. In this study,...
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Vitrification, in vitro fertilization, and development of Atg7 deficient mouse oocytes

Bang S, Lee GK, Shin H, Suh CS, Lim HJ

OBJECTIVE: Autophagy contributes to the clearance and recycling of macromolecules and organelles in response to stress. We previously reported that vitrified mouse oocytes show acute increases in autophagy during warming....
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A new mosaic der(18)t(1;18)(q32.1;q21.3) with developmental delay and facial dysmorphism

Choi YJ, Shin E, Jo TS, Moon JH, Lee SM, Kim JH, Oh JW, Kim CR, Seol IJ

We report the case of a 22-month-old boy with a new mosaic partial unbalanced translocation of 1q and 18q. The patient was referred to our Pediatric Department for developmental delay....
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The potential role of granulosa cells in the maturation rate of immature human oocytes and embryo development: A co-culture study

Jahromi BN, Mosallanezhad Z, Matloob N, Davari M, Ghobadifar MA

OBJECTIVE: In order to increase the number of mature oocytes usable for intracytoplasmic sperm injection (ICSI), we aimed to investigate the effect of co-culturing granulosa cells (GCs) on human oocyte...
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Cryptic Insertion of the BCR Gene at 9q34 in Philadelphia-Negative Chronic Myelogenous Leukaemia

Kim HJ, Ji M, Kim H, Moon HW, Hur M, Yun YM

Chronic myelogenous leukaemia (CML) is a myeloproliferative neoplasm that is almost always characterised by the presence of t(9;22)(q34;q11.2). Approximately 5% to 10% of CML patients lack cytogenetic evidence of t(9;22)(q34;q11.2)...
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Evaluation of the genotoxicity of ginseng leaf extract UG0712

Kim JY, Ri Y, Do SG, Lee YC, Park SJ

Although ginseng (genus Panax) leaf extract contains high concentrations of bioactive constituents, its effects have been reported in few preclinical studies, and information regarding its toxicity is not sufficient to...
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In vitro growth of mouse preantral follicles: effect of animal age and stem cell factor/insulin-like growth factor supplementation

Jee BC, Kim JH, Park DH, Youm H, Suh CS, Kim SH

OBJECTIVE: To determine whether animal age impacts in vitro preantral follicle growth. Effects of hCG, stem cell factor (SCF), and/or insulin-like growth factor (IGF) supplementation in growth medium were also...
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Ring Chromosome 5 in Acute Myeloid Leukemia Defined by Whole-genome Single Nucleotide Polymorphism Array

Huh J, Mun YC, Chung WS, Seong CM

Chromosomes forming a corresponding ring cannot be clearly defined by conventional cytogenetics or FISH. Karyotypic analyses using whole-genome single nucleotide polymorphism arrays (SNP-A) may result in the identification of previously...
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Function of the pentose phosphate pathway and its key enzyme, transketolase, in the regulation of the meiotic cell cycle in oocytes

Kim Y, Kim EY, Seo YM, Yoon TK, Lee WS, Lee KA

OBJECTIVE: Previously, we identified that transketolase (Tkt), an important enzyme in the pentose phosphate pathway, is highly expressed at 2 hours of spontaneous maturation in oocytes. Therefore, this study was...
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Sole Trisomy 22 Not Associated with inv(16) in Myelodysplastic Syndrome

Hahm C, Hwang Y, Mun YC, Seong CM, Chung WS, Huh J

Trisomy 22 is closely associated with inv(16) or t(16;16) and could be a marker of cryptic rearrangement of CBFB/MYH11 in acute myeloid leukemia (AML). Trisomy 22 not associated with CBFB/MYH11...
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