Korean J Fertil Steril.
1998 Apr;25(1):65-70.
The Effects of Glutamine on Blastulation of Human Embryos on Vero Cells In Vitro
Abstract
- This study was conducted to investigate the effects of Tissue Culture Medium 199 (TCM) and Dulecco's Modified Eagle Medium (DMEM) on the blastulation and grade of human oocytes on Vero cells in vitro. A cohort of 79 and 93 oocytes in metaphase II stage were used in TCM 199 and DMEM respectively. No differences were found in the nurser of oocytes showing two-pronuclei between TCM (82.3%) and DMEM (86.0%). The number of fertilized oocytes reaching the blastocyst was not significant in TCM (60.0%) and DMEM (63.1%).4 total of 89 blastocysts were categorized into the four grades (BG1, BG2, BG3 and early) depending on their morphology. The number of embryos achieving the blastocyst grade 1 (BG1) was significantly higher (p<0.05) in DMEM (50.8%) than TCM (15.0%). It is concluded that cultured oocytes in DMEM with glutamine on Vero cells should be significantly increased BG1.
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- The Effect of Coculture on In Vitro Fertilization of Oocytes and Development of Early Stage Embryos in Mice
- The Effects of Vero Cell Coculture on Mouse Oocyte Maturation and Embryo Development in Vitro
- Effect of Human Oviduct Epithelial Cells and Vero Cell on Early Mouse Embryonal Development In Vitro
- Studies on development of serum-free conditioned media using Vero cells and DMEM with controlled concentration of glucose and pyruvate
- Vero Cell Co-culture Counteracts the Detrimental Effects of Hydrosalpinx Fluid on the Development of Mouse Embryos in vitro
