A Comparison Study of Metaphase Analysis of Chromosomal Aberration and Flow Cytometric Assessment of Radiation- induced Apoptosis in Human Peripheral Lymphocytes

Bom, Hee Seung; Lee, Seung Yeon; Lee, Sang Ku; Min, Jung Jun; Jeong, Hwan Jeong; Song, Ho Cheon; Kim, Ji Yeul; Shin, Jong Hee; Suh, Sun Pal; Rhang, Dong Wook

Abstract

PURPOSE: Radiation-induced chromosomal damage and apoptosis were compared in human lymphocytes.
MATERIALS AND METHODS
Peripheral lymphocytes from 10 normal volunteers (6 males, 4 females, age range 23~41 years) were irradiated by gamma rays from a cell irradiator. Doses of irradiation were 0 (control), 0.18, 2, 5, 10, 20 and 25 Gy. Irradiated lymphocytes were examined by metaphase analysis for chromosomal aberrations and by flow cytometry for apoptosis.
RESULTS
of both studies were compared according to dose.
RESULTS
Number of dicentric and ring chromosomes (D+R) was 0.5+/-0.53 at baseline, which was significantly increased after radiation according to the dose. The fraction of cells showing annexin V-fluore-scein isothiocyanate uptake was 0.55+/-0.39%, which increased to 3.58+/-1.85% by 2 Gy irradiation, and then decreased. The fraction of cells showing propidium iodide (PI) uptake was 0.52+/-0.12%, which significantly increased according to dose (upto 15.64+/-5.99% by 20 Gy irradiation). D+R and PI uptake were well correlated (r=0.84, p<0.001).
CONCLUSION
Radiation-induced chromosomal aberration was correlated to nuclear uptake of PI, a marker of late apoptosis.