Korean J Otolaryngol-Head Neck Surg.  2001 May;44(5):466-469.

The Difference between the Concentrations of Arachidonic Acid Metabolites in the Cholesterol Granuloma and Granulation Tissues in Chronic Otitis Media

• Jang YJ
• Kim HG
• Oh JK
• Rhee CK

Abstract

BACKGROUND AND OBJECTIVES
Both the granulation tissue and cholesterol granuloma can erode the surrounding bone and ossicles. However, the etiology of bone resorption in the granulation tissue and cholesterol granuloma has not been evident. The aim of this study was to assay the concentrations of arachidonic acid metabolites (AAMs) in cholesterol granuloma and the mastoid granulation tissue in order to better understand the possible role of AAMs in the pathophysiology of cholesterol granuloma in comparison with the granulation tissue. MATERIALS AND METHOD: Cholesterol granuloma tissues were obtained from eight patients who had underwent tympanomastoidectomy. Granulation tissues, which served for comparison, were taken from 12 patients who had underwent tympanomastoidectomy. Tissue concentrations of prostagladin (PG)E2, 6-keto-PGF1alpha, leukotriene (LT)C4, LTD4, LTE4, 15-hydro xyeicosatetraenoic acid (HETE), 12-HETE, 5-HETE, and thromboxane (TXB)2 were calculated using high performance liquid chromatography and compared between cholesterol granuloma and granulation tissue. RESULT: The level of 12-HETE was higher in cholesterol granuloma than in the granulation tissue. Among the PGs, the tissue concentration of PGE2 was particularly high in cholesterol granuloma than in the granulation tissue. LTD4 was the only LT detectable in cholesterol granuloma. In comparison to the AAMs in cholesterol granuloma, the lipoxygenase pathway products such as 12-HETE, 15-HETE, and 5-HETE were present in lower concentrations in the granulation tissue. LT was undetectable in the granulation tissues.
CONCLUSION
Our results suggest that cholesterol granuloma and the granulation tissue is different not only in terms of histology, but also in terms of biochemical properties such as arachidonic acid metabolism.

Keyword

Arachidonic acids; cholesterol granuloma; granulation tissue

MeSH Terms

12-Hydroxy-5,8,10,14-eicosatetraenoic Acid
Arachidonic Acid*
Arachidonic Acids
Bone Resorption
Cholesterol*
Chromatography, Liquid
Cytochrome P-450 CYP1A1
Dinoprostone
Granulation Tissue*
Granuloma*
Humans
Leukotriene D4
Leukotriene E4
Lipoxygenase
Mastoid
Metabolism
Otitis Media*
Otitis*
12-Hydroxy-5,8,10,14-eicosatetraenoic Acid
Arachidonic Acid
Arachidonic Acids
Cholesterol
Cytochrome P-450 CYP1A1
Dinoprostone
Leukotriene D4
Leukotriene E4
Lipoxygenase