Korean J Urol.  1991 Feb;32(1):1-8.

The study on the histopathologic changes in chorioallantoic membrane of the fertilized chicken eggs where the MBT-2 mice bladder cancer and the human bladder cancer tissue were implanted and chemosensitivity test for anticancert drugs

• Bahk JY
• Chung KH
• Jung SH
• Kim JT
• Kim SH

Abstract

The MBT-2 mice bladder cancer tissues and the human bladder cancer tissues were implanted on the chorioallantoic membrance(CAM) of the immune deficient fertilized chicken eggs and the histopathologic changes of the CAM and gross morphologic changes of the implanted cancer tissues on CAM ere studied. The chemosensitivity tests using chicken CAM were performed for the 4 human bladder cancer tissues to mitomycin C, thiotepa and adriamycin. With this study, the following results were obtained: 1. The observation of the blood vessel on the chorioallantoic membrane was possible from the post-incubation 6th day group, but for the implantation of the cancer tissues, the blood vessels from the post-incubation 8th day group was appropriate. 2. The budding oif the host capillary vessel to the implanted cancer tissue were observed from the post-implantation second day. 3. The size of the post-implantation 7th day cancer tissues were varied from 2.3 to 9.2 folds to the size of the implantation day. 4. The total failure rate in experiment within post-operative 3rd day were 71.3 percent and the total failure rates in group who had the damage on the chorioallantoic membrance during operation was 82.5 percent. The failure rate of the experiment was declined acutely after post-operative 4th day. 5. The salvage of the eggs could be maintained until post-operative 7th day in 28.1 percent among chemosensitivity test group. 6. The 4 bladder cencer tissues which had the chemosensitivity test showed 1.6 to 7.1 fold growth to the inital implanted size and this meant resistance to the test drugs and these results were corresponded with clinical course.

Keyword

Fertillized chicken egg; Chorioallantoic membrane assay; In vivo chemosensitivity test

MeSH Terms

Animals
Blood Vessels
Capillaries
Chickens*
Chorioallantoic Membrane*
Doxorubicin
Eggs*
Humans*
Mice*
Mitomycin
Ovum*
Thiotepa
Urinary Bladder Neoplasms*
Urinary Bladder*
Doxorubicin
Mitomycin
Thiotepa