A Study on the Cell Surface Antigens of Bladder Carcinoma by Means of Monoclonal Antibodies

Min, Kweon Sick; Yoon, Jin Han; Yoon, Jong Byung

Abstract

This study was carried out to investigate the localization and change of the cell surface antigens, such as URO-9(Om5) and URO-10 (T43), in transitional cell carcinomas of bladder. The subjects used were normal bladder mucosae of 10 autopsy cases of fetus and 10 biopsy cases of child or adult patients with disease of other organs as controls, and neoplastic tissue of 15 cases of patient with transitional cell in carcinoma of bladder. Avidin-Biotin complex(ABC) kits(Cambridge Research Lab.) and URO-9 and URO-10 monoclonal antibodies were used and the data observed were analyzed. The results obtained were summarized as follows: 1. URO-9 expression was none of 10 fetal bladder mucosae and 3 or 10 normal bladder mucosae and URO-10 expression was none showed. 2. Of 15 cases with transitional cell carcinoma of bladder, URO-9 expression was 5, of which 1 was Ta grade II, 1 T1 grade II and the rest T2 grade III. URO-10 expression was 10, of which I was T1 grade III, 3 T2 grade III, 3 T3 grade III and 3 T4 grade III, of 15 cases of transitional cell carcinoma of bladder. As above results, the transitional cell carcinoma of bladder in low grade and low stage have tendency to express URO-9 monoclonal antibody but not URO-10 monoclonal antibody, whereas the transitional cell carcinoma of bladder in high grade and high stage were inclined to express URO-10 monoclonal antibody with variable expression of URO-9 monoclonal antibody.