J Korean Med Sci.  1994 Feb;9(1):47-51. 10.3346/jkms.1994.9.1.47.

A monoclonal antibody to cell surface antigen of human thymic epithelial cell

• Chung DH
• Bae YM
• Shin HY
• Ahn HS
• Song HG
• Park WS
• Park SH
• Lee SK

• KMID: 1422186
• DOI: http://doi.org/10.3346/jkms.1994.9.1.47

Abstract

The cell surface molecule identified by a monoclonal antibody(TE-1) to human thymic epithelial cell showed the specificity for thymic epithelial cells of both the cortex and medulla. TE-1 reacted with the epithelial cells of normal thymus and thymoma in fresh frozen tissues. The antigen recognized by TE-1 was mostly confined to the cell surface membrane and arranged in reticular network with long processes between thymocytes. On immunohistochemical analysis, TE-1 did not recognize normal epithelial cells of the uterine cervix, skin and stomach, and neoplastic cells of squamous cell carcinoma and gastric adenocarcinoma, all of which were stained with anti-cytokeratin monoclonal antibody. Among the tumor cell lines tested with flow cytometry, most of epithelial and all of hematopoietic cell origin were not labeled with TE-1. In summary, TE-1 appears to be a monoclonal antibody against a surface antigen of human thymic epithelial cell that is immunohistologically different from known epithelial cell surface antigens reported so far.

MeSH Terms

Animals
Antibodies, Monoclonal/biosynthesis/*immunology
Antibody Specificity
Antigens, Surface/*immunology
Epithelium/immunology
Fluorescent Antibody Technique
Humans
Immunoenzyme Techniques
Immunoglobulin G/immunology
Immunoglobulin Isotypes/immunology
Mice
Mice, Inbred BALB C
Neoplasms/immunology
Thymoma/immunology
Thymus Gland/*immunology
Thymus Neoplasms/immunology
Tumor Cells, Cultured
Antibodies, Monoclonal
Antigens, Surface
Immunoglobulin G
Immunoglobulin Isotypes