Korean J Anesthesiol.  2005 Dec;49(6):842-846. 10.4097/kjae.2005.49.6.842.

Effect of Etomidate on N-methyl-D-aspartate Induced Neurotoxicity in Rat Cortical Neurons

• Lee YM
• Lee EH
• Kim JU
• Lee HJ
• Hwang BM
• Shin JW
• Ku SW

• KMID: 1894324
• DOI: http://doi.org/10.4097/kjae.2005.49.6.842

Abstract

BACKGROUND: The present investigation was undertaken to evaluate the neuroprotective effect of etomidate against N-methyl-D- aspartate (NMDA) induced neurotoxicity in rat cortical neurons by measuring lactate dehydrogenase (LDH).
METHODS
The cerebral cortical neurons of fetal rat were grown for 12 days in dissociated cell culture. They were divided into four groups: first group acted as control with no drug administration and other groups treated with either NMDA 100microM or Etomidate (ET) 10microM + NMDA 100microM or ET 100microM + NMDA 100microM After 24 hrs, cell death was assessed by morphology under the light microscope and quantified by measuring the LDH in the culture media.
RESULTS
In the light microscopic findings, the intact cortical neurons were increased in the ET groups. NMDA-induced LDH production also significantly suppressed in ET group (P<0.05). There were no significant difference between the ET 10microM and 100microM groups.
CONCLUSIONS
These results suggest that the etomidate has protective effect on the cultured rat cortical neurons against NMDA-induced neurotoxicity.

Keyword

etomidate; lactate dehydrogenase (LDH); neurotoxicity; N-methyl-D-aspartate (NMDA)

MeSH Terms

Animals
Aspartic Acid
Cell Culture Techniques
Cell Death
Culture Media
Etomidate*
L-Lactate Dehydrogenase
N-Methylaspartate*
Neurons*
Neuroprotective Agents
Rats*
Aspartic Acid
Culture Media
Etomidate
L-Lactate Dehydrogenase
N-Methylaspartate
Neuroprotective Agents