Tuberc Respir Dis.  2002 Jan;52(1):54-61. 10.4046/trd.2002.52.1.54.

A Canine Model of Tracheal Stenosis Using Nd-YAG Laser

• Kim JG
• Suh GY
• Chung MP
• Kwon OJ
• Suh SW
• Kim HJ

• KMID: 1877017
• DOI: http://doi.org/10.4046/trd.2002.52.1.54

Abstract

BACKGROUND: Tracheal stenosis is an urgent but uncommon disease. Therefore, primary care clinicians have limited clinical experience. Animal models of a tracheal stenosis can be used conveniently for the learning, teaching, and developing new diagnostic and therapeutic modalities for tracheal stenosis. Recently, a canine model of a tracheal stenosis was developed using a Nd-YAG laser. To describe the methods and results of developed animal model, we performed this study.
METHODS
Six Mongrel dogs were generally anesthetized and the anterior 180 degree of tracheal cartilage of the animal was photo-coagulated using a Nd-YAG laser. The animals were bronchoscopically evaluated every week for 4 weeks and a pathologic evaluation was also made.
RESULTS
Two weeks after the laser coagulation, the trachea began to stenose and the stenosis progressed through 4 weeks. All animals suffered from shortness of breath, wheezing, and weight loss in the 3 weeks after the laser treatment, and two died of respiratory failure just before the fourth week. The gross pathologic findings showed the loss of cartilage and a dense fibrosis, which resulted in a fibrous stricture of the trachea. Microscopy also showed that the fibrous granulation tissue replaced destroyed cartilage.
CONCLUSION
The canine model can assist in the understanding and development of new diagnostic and therapeutic modalities for tracheal stenosis.

Keyword

Animal model; Upper airway Obstruction; Bronchoscopy

MeSH Terms

Animals
Bronchoscopy
Cartilage
Constriction, Pathologic
Dogs
Dyspnea
Fibrosis
Granulation Tissue
Laser Coagulation
Lasers, Solid-State*
Learning
Microscopy
Models, Animal
Primary Health Care
Respiratory Insufficiency
Respiratory Sounds
Trachea
Tracheal Stenosis*
Weight Loss