Korean J Obstet Gynecol.
2007 Mar;50(3):429-438.
Association of the vascular endothelial damage and estrogen, progesterone
- Affiliations
-
- 1Department of Obstetrics and Gynecology, College of Medicine, Ewha Womans' University, Seoul, Korea. kkyj@ewha.ac.kr
- 2Department of Physiology, College of Medicine, Ewha Womans' University, Seoul, Korea.
- 3Ewha Medical Research Center, College of Medicine, Ewha Womans' University, Seoul, Korea.
- 4Ewha Global Challenge for Medicine Ewha Womans' University, Seoul, Korea.
Abstract
OBJECTIVE
The aim of this study were to examine the serum level of estradiol, estriol, progesterone, oxidized LDL in preeclamtic patients and to evaluate the protective effects of estrogen and progesterone against lysophosphatidylcholine (LPC) induced cell death in Human umbilical vein endothelial cells (HUVECs).
METHODS
We analysed the serum level of estradiol, estriol, progesterone, oxidized LDL in patients with preeclampsia and control. We used LPC to induce cell death in HUVECs. For cytotoxic assay, we did LDL assay for cell death and Resazurin assay for cell viability. HUVECs were exposed to various concentrations of LPC, LPC+estrogen, LPC+progesterone and we did cytotoxic assay.
RESULTS
The serum estradiol, estriol were lower in the preeclamptic patients (P<0.05). Oxidized LDL were higher in the preeclamptic patients(P<0.05). LPC induced cell death in a concentration-dependant manner. Estrogen or progesterone inhibited LPC-induced cell death in a concentration-dependant manner (P<0.05).
CONCLUSION
Estrogen and progesterone attenuated LPC-induced cytotoxicity. The results suggest that Oxidized LDL induced endothelial damage in preeclampsia may be induced by low serum estradiol, estriol and progesterone levels and prevented by estrogen and progesterone addition.
Keyword
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Immunohistochemical studies on the rate of positive reaction of estrogen receptors and progesterone receptors in the breast diseases
- Regulation of Estrogen Receptor and Progesterone Receptor Expression by Estrogen and Progesterone/Progestogen in Human Umbilical Vein Endothelial Cells
- Estrogen and Progesterone receptors in Meningioma
- Estrogen Rather Than Progesterone Cause Constipation in Both Female and Male Mice
- Study for estrogen receptor and progesterone receptor of colorectal carcinoma
