Yonsei Med J.  2014 Sep;55(5):1395-1399. 10.3349/ymj.2014.55.5.1395.

Passive Skeletal Muscle Excursion after Tendon Rupture Correlates with Increased Collagen Content in Muscle

• Koh IH
• Kang HJ
• Jeon SW
• Park JH
• Choi YR

• KMID: 1799508
• DOI: http://doi.org/10.3349/ymj.2014.55.5.1395

Abstract

PURPOSE
This study was designed to measure time-dependent changes in muscle excursion and collagen content after tenotomy, and to analyze the correlation between muscle excursion and collagen content in a rabbit model.
MATERIALS AND METHODS
Twenty-four rabbits underwent tenotomy of the second extensor digitorum longus (EDL) muscles on the right legs and were randomly assigned to three groups based on the period of time after tenotomy (2, 4, and 6 weeks). The second EDL muscles on left legs were used as controls. At each time after tenotomy, passive muscle excursion and collagen content, determined by hydroxyproline content, were measured bilaterally, and the ratio of each value to the normal one was used.
RESULTS
The mean ratio of muscle excursion after tenotomy to the value of the control decreased in a time-dependent fashion: 92.5% at 2 weeks, 78.6% at 4 weeks, and 55.1% at 6 weeks. The mean ratio of hydroxyproline content in muscle to the value of the control increased in a time-dependent fashion: 119.5% at 2 weeks, 157.3% at 4 weeks, and 166.6% at 6 weeks. There was a significant negative correlation between the ratio of hydroxyproline content in muscle after tenotomy to the control values and the ratio of muscle excursion after tenotomy to the control values (r=-0.602, p=0.002).
CONCLUSION
The decrease in muscle excursion seems to correlate with the increase in collagen content in the muscle in a time-dependent fashion following tenotomy.

Keyword

Tenotomy; muscle excursion; collagen content

MeSH Terms

Animals
Collagen/*metabolism
Hydroxyproline/metabolism
Muscle, Skeletal/*metabolism
Rabbits
Tendon Injuries/*metabolism
Tendons
Tenotomy
Time Factors
Collagen
Hydroxyproline

Figure

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  Fig. 1 Time-dependent change in the ratio of passive muscle excursion to normal value (%) following tenotomy of the second extensor digitorum longus muscle.

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  Fig. 2 Time-dependent changes in the ratios of hydroxyproline (collagen) content to the normal value (%) following tenotomy of the second extensor digitorum longus muscle.

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