J Korean Med Sci.  2014 Jan;29(1):106-109. 10.3346/jkms.2014.29.1.106.

Correlation between Positive Rate and Number of Biopsy Samples on Urease Test in Childhood Helicobacter pylori Infection

• Seo JH
• Park JS
• Yeom JS
• Lim JY
• Park CH
• Woo HO
• Baik SC
• Lee WK
• Cho MJ
• Rhee KH
• Youn HS

• KMID: 1796922
• DOI: http://doi.org/10.3346/jkms.2014.29.1.106

Abstract

To identify the correlation between the number of gastric biopsy samples and the positive rate, we compared the results of urease test using one and three biopsy samples from each 255 children who underwent gastroduodenoscopy at Gyeongsang National University Hospital. The children were divided into three age groups: 0-4, 5-9, and 10-15 yr. The gastric endoscopic biopsies were subjected to the urease test. That is, one and three gastric antral biopsy samples were collected from the same child. The results of urease test were classified into three grades: Grade 0 (no change), 1 (6-24 hr), 2 (1-6 hr), and 3 (<1 hr). The positive rate of urease test was increased by the age with no respect to the number of gastric biopsy samples (one biopsy P = 0.001, three biopsy P < 0.001). The positive rate of the urease test was higher on three biopsy samples as compared with one biopsy sample (P < 0.001). The difference between one and three biopsy samples was higher in the children aged 0-9 yr. Our results indicate that the urease test might be a more accurate diagnostic modality when it is performed on three or more biopsy samples in children.

Keyword

Urease Test; Helicobacter pylori Infection; Age; Histopathology

MeSH Terms

Adolescent
*Biopsy
Child
Child, Preschool
Duodenoscopy
Female
Helicobacter Infections/*diagnosis
Helicobacter pylori/pathogenicity
Humans
Infant
Infant, Newborn
Male
Pyloric Antrum/microbiology
Urease/*analysis
Urease

Figure

• 

  Fig. 1 The difference in the positive rate of urease test between one and three biopsy samples in all the age groups. The positive rate of urease test was increased with the age with no respect to the number of gastric biopsy samples (one biopsy P = 0.001, three biopsy P < 0.001). The positive rate of the urease test was higher on three biopsy samples as compared with one biopsy sample (P < 0.05). GU1, 32.2%; GU3, 40.1%.

• 

  Fig. 2 The cumulative percentage of positive rate depending on the age and the number of biopsy samples. The degree of the correlation between the cumulative positive rate and the incubation time was higher on three biopsy samples as compared with one biopsy sample. GU1, Spearman R = 0.931, P < 0.001; GU3, Spearman R = 0.907, P < 0.001.

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