Yonsei Med J.  2009 Dec;50(6):832-837. 10.3349/ymj.2009.50.6.832.

The Protective Effect of Sodium Hyaluronate on the Cartilage of Rabbit Osteoarthritis by Inhibiting Peroxisome Proliferator-Activated Receptor-Gamma Messenger RNA Expression

• Zhou JL
• Liu SQ
• Qiu B
• Hu QJ
• Ming JH
• Peng H

• KMID: 1777099
• DOI: http://doi.org/10.3349/ymj.2009.50.6.832

Abstract

PURPOSE
The purpose of this study was to study the protective effect and influence of sodium hyaluronate (Na-HA) on mRNA expression of peroxisome proliferators-activated receptor gamma (PPAR-gamma) in cartilage of rabbit osteoarthritis (OA) model.
MATERIALS AND METHODS
Forty eight white rabbits were randomly divided into A, B, and C groups. Group A was normal control group, B and C groups underwent unilateral anterior cruciate ligament transection (ACLT). The rabbits in group B were injected normal saline after ACLT; and Group C received intraarticular1% sodium hyaluronate (HA) injection 5 weeks after surgery, 0.3 mL once a week. At 11th week after surgery, all the rabbits were sacrificed. The cartilage changes on the medial femoral condyles were graded separately. Cartilage sections were stained with safranin-O and HE, and messenger RNA (mRNA) expression of PPAR-gamma was detected by using real time polymerase chain reaction (Real Time-PCR).
RESULTS
Cartilage degeneration in group B was significantly more severe than in A and C injection group. The grey value of Safranin-O of B group was higher than A and C groups. Expression of PPAR-gamma mRNA in group B was higher than group A and C.
CONCLUSION
This study shows that Na-HA has a protective effect on articular cartilage degeneration, and the inhibitory effect on the PPAR-gamma mRNA expression may be one of therapeutic mechanism of Na-HA.

Keyword

Sodium hyaluronate; Peroxisome proliferators-activated receptor gamma; cartilage; osteoarthritis

MeSH Terms

Animals
Cartilage/*drug effects/*metabolism
Gene Expression/drug effects/genetics
Hyaluronic Acid/*pharmacology/therapeutic use
Microscopy
Osteoarthritis/*drug therapy/metabolism
PPAR gamma/*genetics
RNA, Messenger/*genetics
Rabbits
Random Allocation
Reverse Transcriptase Polymerase Chain Reaction
Viscosupplements/*pharmacology/therapeutic use

Figure

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  Fig. 1 Representative sections of cartilage from the femoral condyles (HE staning). (A) Cartilage of normal showing no evident changes. (B) NS group showing loss of height of articular cartilage, increased flaking of cartilage, clefts deep to the radial zone, loss of coumnization of cellular distribution, and increased areas of hypocellularity. (C) HA could protect articular cartilage from loss of height, and decrease the flaking of cartilage. NS, normal sodium treated group; HA, sodium hyaluronate treated group.

• 

  Fig. 2 Representative sections of cartilage from the femoral condyles (Safranin-O staining). (A) Cartilage of normal showing an intact profile, and there is no evident loss of proteoglycan (PGs). (B) NS group showing severe loss of PGs, and clefts deep to the radial zone. (C) HA could inhitit the loss of PGs in cartilage matrix.

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  Fig. 3 Stand curve of GAPDH in fluorescent quantitative PCR. PCR, polymerase chain reaction; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.

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  Fig. 4 Amplification plot of PPAR-γ and GAPDH. PPAR-γ, peroxisome proliferators-activated receptor gamma; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.

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  Fig. 5 Dissociation curve of PPAR-γ and GAPDH. PPAR-γ, peroxisome proliferators-activated receptor gamma; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.

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  Fig. 6 Real Time PCR for the relative expression of PPAR-γ mRNA in cartilage. There was significant difference between NS and NOR, HA cartilage (p < 0.05), and no difference between NOR and HA cartilage (p > 0.05). PCR, polymerase chain reaction; PPAR-γ, peroxisome proliferators-activated receptor gamma; mRNA, messenger RNA; NS, normal sodium treated group; NOR, normal control group; HA, sodium hyaluronate treated group.

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