Korean J Anesthesiol.  2011 Oct;61(4):327-331. 10.4097/kjae.2011.61.4.327.

Effects of sevoflurane on neuronal cell damage after severe cerebral ischemia in rats

• Park HP
• Jeong EJ
• Kim MH
• Hwang JW
• Lim YJ
• Min SW
• Kim CS
• Jeon YT

• KMID: 1766775
• DOI: http://doi.org/10.4097/kjae.2011.61.4.327

Abstract

BACKGROUND
The aim of this study was to investigate the neuroprotective effects of sevoflurane after severe forebrain ischemic injury. We also examined the relationship between the duration of ischemia and neuronal cell death.
METHODS
Male Sprague-Dawley rats (300-380 g) were subjected to 6 (each n = 6) or 10 min (each n = 10) of near-complete forebrain ischemia while anesthetized with either 50 mg/kg of zoletil given intraperitoneally or inhaled sevoflurane (2.3%). Ischemia was induced by bilateral common carotid artery occlusion plus hemorrhagic hypotension (26-30 mmHg). Histologic outcomes were measured 7 days after ischemia in CA1 pyramidal cells of the rat hippocampus.
RESULTS
The mean percentage of necrotic cells in the hippocampal CA1 area decreased in the sevoflurane group compared to the zoletil group (25% vs. 40% after 6 min ischemia, respectively: P = 0.004 and 44% vs. 54% after 10 min of ischemia, respectively P = 0.03). The percentage of apoptotic cells was similar in all groups. The percentage of necrotic cells in each anesthetic groups was significantly higher in the 10 min ischemia group compared to the 6 min ischemia group (P = 0.004 in the sevoflurane group, P = 0.03 in the zoletil group).
CONCLUSIONS
The present data show that sevoflurane has neuroprotective effects in rats subjected to near-complete cerebral ischemia. Longer duration of ischemia is associated with more neuronal injury when compared to ischemia of shorter duration.

Keyword

Brain ischemia; Hippocampus; Inhalation anesthetics; Neuron

MeSH Terms

Anesthetics, Inhalation
Animals
Brain Ischemia
Carotid Artery, Common
Drug Combinations
Hippocampus
Humans
Hypotension
Ischemia
Male
Methyl Ethers
Neurons
Neuroprotective Agents
Prosencephalon
Pyramidal Cells
Rats
Rats, Sprague-Dawley
Tiletamine
Zolazepam
Anesthetics, Inhalation
Drug Combinations
Methyl Ethers
Neuroprotective Agents
Tiletamine
Zolazepam