Yonsei Med J.  2009 Aug;50(4):564-568. 10.3349/ymj.2009.50.4.564.

Polymorphism of a COLIA1 Gene Sp1 Binding Site in Korean Women with Pelvic Organ Prolapse

Affiliations
  • 1Department of Obstetrics and Gynecology, Yonsei University College of Medicine, Seoul, Korea. swbai@yuhs.ac
  • 2Department of Laboratory Medicine, Yonsei University College of Medicine, Seoul, Korea.
  • 3Department of Pathology, Yonsei University College of Medicine, Seoul, Korea.

Abstract

PURPOSE
To evaluate the possible influence of G-->T substitution at the Sp1-binding site of the COLIA1 gene on the risk of pelvic organ prolapse (POP). MATERIALS AND METHODS: The study group consisted of 15 women with advanced stage POP. Fifteen control subjects with uterine myomas among the postmenopausal women were matched for age and parity. DNA was obtained from peripheral blood leukocytes. The fragments of the first intron of the COLIA1 gene were amplified by real time polymerase chain reaction. The polymorphism was identified using LightCycler Technology with hybridization probes. Sequencing reactions were performed on each template using commercial primer. RESULTS: Two groups had no significant difference in medical history, surgical, and smoking history. The homozygous peaks in two groups were noted at 57degrees C on melting curve analysis. Sequencing reactions confirmed the G/G alleles in the 30 specimens tested. We could not find any polymorphism at the Sp1-binding site in COLIA1 gene with advanced stage POP. Statistical significance was considered to be p < .05. CONCLUSION: The polymorphism of the Sp1-binding site in the COLIA1 gene did not contribute to the development of POP in Korea.

Keyword

Polymorphism; prolapse

MeSH Terms

Aged
Asian Continental Ancestry Group/genetics
Binding Sites/genetics
Collagen Type I/*genetics
Female
Genetic Predisposition to Disease
Humans
Middle Aged
Pelvic Organ Prolapse/*genetics
Polymerase Chain Reaction
Polymorphism, Genetic/*genetics
Sp1 Transcription Factor/*metabolism

Figure

  • Fig. 1 Melting peak analysis of the Sp1 binding site of COLIA1 gene in the study group on the LightCycler instrument.

  • Fig. 2 The Sp1 binding site of COLIA1 gene in the control group by melting peak analysis.

  • Fig. 3 Sequencing of the Sp1 binding site of the COLIA1 gene. *The guanine residue of Sp1-binding site in COLIA1 gene.


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