Yonsei Med J.  2000 Apr;41(2):185-189. 10.3349/ymj.2000.41.2.185.

The effective concentration and exposure time of mitomycin-C for the inhibition of lens epithelial cell proliferation in rabbit eyes

• You YS
• Lim SJ
• Chung HS
• Lee YG
• Kim CY
• Hong YJ

• KMID: 1758507
• DOI: http://doi.org/10.3349/ymj.2000.41.2.185

Abstract

The proliferation of residual lens epithelial cells following cataract surgery is assumed to be a major cause of posterior capsular opacification. To assess the efficacy of mitomycin-C in preventing posterior capsular opacification, we determined the effective concentration and exposure time of mitomycin-C in inhibiting rabbit lens epithelial cell proliferation. The fourth-passaged rabbit lens epithelial cells were maintained for one day and then exposed to mitomycin-C for 1, 2, 3, and 5 minutes, respectively. There were 9 different plating concentrations of mitomycin-C with two-fold serial dilution. The maintenance of the phenotypic properties of lens epithelial cells was confirmed by continuous transcription of lambda-crystalline mRNA determined by reverse transcription-polymerase chain reaction and the polymorphism of the restriction fragment. Cell proliferation was assayed with 3H-thymidine incorporation into DNA. The fourth-passaged cells maintained the expression of lambda-crystalline mRNA, suggesting that they are phenotypically authentic lens epithelial cells. The effective concentrations and exposure time of mitomycin-C were 0.1 mg/ml for 1 minute and 2 minutes, and 0.025 mg/ml for 2 minutes. By these results, we postulated that mitomycin-C at relatively short incubation times could be clinically used for prevention of posterior capsular opacification after cataract surgery.

Keyword

e-crystalline mRNA; lens epithelial cell; mitomycin-C; posterior capsular opacity

MeSH Terms

Animal
Antibiotics, Antineoplastic/pharmacology*
Cell Division/drug effects
Cells, Cultured
DNA/biosynthesis
Dose-Response Relationship, Drug
Epithelial Cells/drug effects
Epithelial Cells/cytology
Lens, Crystalline/drug effects*
Lens, Crystalline/cytology
Mitomycin/pharmacology*
Rabbits
Time Factors