J Breast Cancer.  2007 Jun;10(2):114-119. 10.4048/jbc.2007.10.2.114.

The gamma-Synuclein Expression in Breast Cancer and its Correlation with the Expression of the HER-2/neu Gene

Affiliations
  • 1Department of Surgery, Hallym University College of Medicine, Seoul, Korea. hhh@hallym.or.kr
  • 2Department of Pathology, Hallym University College of Medicine, Seoul, Korea.

Abstract

PURPOSE: Synuclein has been identified as an important neuroprotein for developing pathologic deposits in Alzheimer's and Parkinson's disease patients. gamma-synuclein is also known as a breast cancer-specific gene 1 thats's not found in normal breast tissues but it has been reported to be overexpressed in breast cancer, ovarian cancer and other tumors. To evaluate the availability of gamma-synuclein expression as a prognostic factor for infiltrative breast cancer, we analyzed its correlation with the clinical parameters and the HER-2/neu gene expression.
METHODS
Two hundred fiffty samples of breast cancer tissues embedded in paraffin and that were obtained from the infiltrative breast cancer patients who were operated in our institution from January 1995 to December 2000 were analyzed with employing the tissue microarray technique. The expression of gamma-synuclein was studied with immunohistochemistry and with using gamma-synulcein antibodies. One hundred thirty one cases that showed favorable staining were selected and studied retrospectively.
RESULTS
Fiffty five% (71/131) of the patients showed gamma-synuclein overexpression. The histopathological findings that significantly correlated with gamma-synuclein overexpression were the number of metastatic lymph nodes (p<0.01) and the cancer stage (p<0.01). Using the same tissue mircoarray, the HER-2/neu gene expression and gamma-synuclein expression also showed statistically significant correlation (p=0.04).
CONCLUSION
gamma-synuclein overexpression showed significant correaltion with lymph node metastasis and cancer stage. It also showed significant relevance with the HER-2/neu gene expression, and that is already known to be a prognostic factor for breast cancer. Therefore gamma-synuclein may be a useful prognostic factor for infiltrative breast cancer and further studies on the its correlation with survival, local recurrence, and distant metastasis should be conducted

Keyword

Invasive ductal carcinoma; gamma-synuclein; HER-2/neu; Immunohistochemistry

MeSH Terms

Antibodies
Breast Neoplasms*
Breast*
gamma-Synuclein*
Gene Expression
Humans
Immunohistochemistry
Lymph Nodes
Neoplasm Metastasis
Ovarian Neoplasms
Paraffin
Parkinson Disease
Recurrence
Retrospective Studies
Synucleins
Antibodies
Paraffin
Synucleins
gamma-Synuclein

Figure

  • Fig 1 Immunohistochemistry of γ-synuclein (A) non-expression of γ-synuclein (B) expression of γ-synuclein (brown color).


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