Int J Stem Cells.  2012 Nov;5(2):115-124.

Tissue Regeneration and Stem Cell Distribution in Adriamycin Induced Glomerulopathy

Affiliations
  • 1Histology Department, Faculty of Medicine, Cairo University, Cairo, Egypt. mahakaah@yahoo.com
  • 2Misr University of Science and Technology, Cairo, Egypt.
  • 3Clinical Pathology Department, Faculty of Medicine, Cairo University, Cairo, Egypt.

Abstract

BACKGROUND AND OBJECTIVES
Glomerulosclerosis develops secondary to various kidney diseases. It was postulated that adriamycin (ADR) induce chronic glomerulopathy. Treatment combinations for one year did not significantly modify renal function in resistant focal segmental glomerulosclerosis (FSGS). Recurrence of FSGS after renal transplantation impacts long-term graft survival and limits access to transplantation. The present study aimed at investigating the relation between the possible therapeutic effect of human mesenchymal stem cells (HMSCs), isolated from cord blood on glomerular damage and their distribution by using ADR induced nephrotoxicity as a model in albino rat.
METHODS AND RESULTS
Thirty three male albino rats were divided into control group, ADR group where rats were given single intraperitoneal (IP) injection of 5 mg/kg adriamycin. The rats were sacrificed 10, 20 and 30 days following confirmation of glomerular injury. In stem cell therapy group, rats were injected with HMSCs following confirmation of renal injury and sacrificed 10, 20 and 30 days after HMSCs therapy. Kidney sections were exposed to histological, histochemical, immunohistochemical, morphometric and serological studies. In response to SC therapy multiple Malpighian corpuscles (MC) appeared with patent Bowman's space (Bs) 10 and 20 days following therapy. One month following therapy no remarkable shrunken glomeruli were evident. Glomerular area and serum creatinine were significantly different in ADR group in comparison to control and SC therapy groups.
CONCLUSIONS
ADR induced glomerulosclerosis regressed in response to cord blood HMSC therapy. A reciprocal relation was recorded between the extent of renal regeneration and the distribution of undifferentiated mesenchymal stem cells.

Keyword

Mesenchymal stem cells; Cord blood; Glomerulosclerosis; Adriamycin

MeSH Terms

Animals
Creatinine
Doxorubicin
Fetal Blood
Glomerulosclerosis, Focal Segmental
Graft Survival
Humans
Kidney
Kidney Diseases
Kidney Transplantation
Male
Mesenchymal Stromal Cells
Rats
Recurrence
Regeneration
Stem Cells
Transplants
Creatinine
Doxorubicin
Glomerulosclerosis, Focal Segmental
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