Korean J Parasitol.  2008 Mar;46(1):45-48. 10.3347/kjp.2008.46.1.45.

Evaluation of the Korean Isolate-1 Tachyzoite Antigen for Serodiagnosis of Toxoplasmosis

• Shin EH
• Kim DH
• Lin A
• Lee JW
• Kim HJ
• Ahn MH
• Chai JY

• KMID: 1720551
• DOI: http://doi.org/10.3347/kjp.2008.46.1.45

Abstract

To evaluate the usefulness of the Korean Isolate-1 (KI-1) antigen for serodiagnosis of toxoplasmosis, antigen profiles of KI-1 tachyzoites were analyzed in comparison with RH tachyzoites by SDS-PAGE and immunoblotting. ELISA was performed on latex agglutination (LA)-positive and negative serum samples using KI-1 and RH antigens. Immunoblotting of the KI-1 antigen showed multiple antigen bands with molecular sizes of 22-105 kDa. Among them, 1 and 6 common bands were noted against a KI-1-infected and a RH-infected human serum, respectively, which represented differences in antigenic profiles between KI-1 and RH tachyzoites. However, all 9 LA-positive human sera were found positive by ELISA, and all 12 LA-negative sera were negative by ELISA; the correlation between the ELISA titers and LA titers was high (r = 0.749). Our results suggest that tachyzoites of KI-1 may be useful for serodiagnosis of human toxoplasmosis.

Keyword

Toxoplasma gondii; Toxoplasmosis; Korean isolate-1 (KI-1); RH strain; Serodiagnosis; Antigen

MeSH Terms

Adolescent
Adult
Animals
Antigens, Protozoan/*blood
Enzyme-Linked Immunosorbent Assay
Female
Humans
Korea
Latex Fixation Tests
Male
Mice
Middle Aged
Rabbits
Serologic Tests
Toxoplasmosis/blood/*diagnosis