Korean J Parasitol.  2012 Sep;50(3):233-238. 10.3347/kjp.2012.50.3.233.

Production and Evaluation of Toxoplasma gondii Recombinant GRA7 for Serodiagnosis of Human Infections

• Selseleh M
• Keshavarz
• Mohebali M
• Shojaee S
• Selseleh M
• Eshragian
• Mansouri F
• Modarressi MH

• KMID: 1387411
• DOI: http://doi.org/10.3347/kjp.2012.50.3.233

Abstract

The precise diagnosis of the acute toxoplasmosis in pregnant women and immunocompromsied patients has critical importance. Most of the commercially available assays use the whole Toxoplasma soluble extract as the antigen. However, the assays currently available for the detection of specific anti-Toxoplasma antibodies may vary in their abilities to detect serum immunoglobulins, due to the lack of a purified standardized antigen. The aim of this study was production and evaluation of the usefulness of the recombinant Toxoplasma gondii GRA7 antigen for the serodiagnosis of Toxoplasma gondii IgM and IgG by ELISA. A total of 70 T. gondii IgM positive sera, 74 T. gondii IgG positive sera, and 60 sera from subjects who were not infected with T. gondii were examined. These sera were shown different absorbance values in ELISA test. To control the specificity of the rGRA7 other parasitic diseases, for example, echinococcosis, malaria, leishmaniasis, fascioliasis, and strongyloidiasis were tested of which none showed positive results. Sensitivity and specificity of the generated recombinant IgG ELISA in comparison with commercial ELISA (com ELISA) were 89% and 90%, and the sensitivity and specificity of the generated recombinant IgM ELISA were 96% and 90%, respectively. The results obtained here show that this antigen is useful for diagnostic purposes.

Keyword

Toxoplasma gondii; recombinant GRA7; serodiagnosis; ELISA

MeSH Terms

Antibodies, Protozoan/*blood
Antigens, Protozoan/*diagnostic use/genetics/*isolation & purification
Enzyme-Linked Immunosorbent Assay/methods
Female
Humans
Immunoglobulin G/blood
Immunoglobulin M/blood
Protozoan Proteins/*diagnostic use/genetics/*isolation & purification
Recombinant Proteins/diagnostic use/genetics/isolation & purification
Sensitivity and Specificity
Toxoplasma/*immunology
Toxoplasmosis/*diagnosis