Yonsei Med J.
2003 Feb;44(1):65-74. 10.3349/ymj.2003.44.1.65.
Identification of Tumor Suppressor Loci on the Long Arm of Chromosome 15 in Primary Small Cell Lung Cancer
- Affiliations
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- 1Department of Internal Medicine and Cancer Metastasis Research Center, Yonsei University College of Medicine, 134 Shinchon-dong, Seodaemun- gu, Seoul 120-752, Korea. sekyukim@yumc.yonsei.ac.kr
- 2Department of Internal Medicine, Brain Korea 21 Project for Medical Sciences, Yonsei University College of Medicine, Seoul, Korea.
- 3Department of Cardiovascular and Thoracic Surgery, Yonsei University College of Medicine, Seoul, Korea.
- 4Department of Pathology, Yonsei University College of Medicine, Seoul, Korea.
- 5The Institute of Chest Diseases, Yonsei University College of Medicine, Seoul, Korea.
- 6Cancer Metastasis Research Center, Yonsei University College of Medicine, Seoul, Korea.
- KMID: 1719071
- DOI: http://doi.org/10.3349/ymj.2003.44.1.65
Abstract
- Small cell lung cancer (SCLC) frequently shows a loss of heterozygosity (LOH) on chromosome 15q. In order to define the commonly affected region on chromosome 15q, we tested 23 primary SCLCs by microsatellite analysis. By analyzing 43 polymorphic microsatellite markers located on chromosome 15q, we found that 14 (60.8%) of 23 tumors exhibited a LOH in at least one of the tested microsatellite markers. Two (14.3%) of the 14 tumors were found to have more than a 50% LOH on chromosome 15q. LOH was observed in five commonly deleted regions on 15q. Of those regions, LOH from D15S1012 to D15S1016 was the most frequent (47.8%). LOH was also observed in more than 20-30% of tumors at four other regions, from D15S1031 to D15S1007, from D15S643 to D15S980, from D15S979 to D15S202, and from D15S652 to D15S642. Four of the 23 tumors exhibited shifted bands in at least one of the tested microsatellite markers. Shifted bands occurred in 3.2% (29 of 914) of the loci tested. Our data suggests the presence of at least five tumor suppressor loci on chromosome 15q in SCLC, and further that these may play an important role in SCLC tumorigenesis.
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