Korean J Thorac Cardiovasc Surg.
2009 Apr;42(2):141-147.
Identification of Tumor Suppressor Genes on Chromosome 21
- Affiliations
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- 1Department of Thoracic and Cardiovascualr Surgery, Kyungpook National University Hospital, College of Medicine, Kyungpook National University, Korea. bay@knu.ac.kr
- 2Department of Biochemistry, School of Medicine, Kyungpook National University, Korea.
- 3Department of Internal Medicine, Kyungpook National University Hospital, College of Medicine, Kyungpook National University, Korea.
Abstract
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BACKGROUND: We performed this study to identify the tumor suppressor genes located in the long arm of chromosome 21 in non-small cell lung cancer.
MATERIAL AND METHOD: The genes of USP25 in 21q11.2, NCAM2, ADAMTS1 in 21q21.2, and Claudin-8 (CLDN8), Claudin-17 (CLDN17) and TIAM1 in 21q22.1 were investigated for their gene expressions, genetic alterations and promoter methylation.
RESULT: The expressions of CLDN8 and CLDN17 were significantly decreased in 7 (L132, H157, H358, H522, H1299, H1703 and HCC2108) of 13 cell lines, and the expression of ADAMTS1 was also significantly reduced in 6 cell lines (A549, SW900, H1299, H1373, H1703 and H1793). There were no genetic alterations by PCR-SSCP and cDNA cloning in the cell lines with a decreased gene. In the cell lines with a decreased gene expression, the mRNA expression was increased significantly with treatment of 5-Aza-CdR.
CONCLUSION
These results suggest that the ADMTS1, CLDN8 and CLDN17 may act as tumor suppressor genes.
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