J Korean Med Sci.  2006 Dec;21(6):979-982. 10.3346/jkms.2006.21.6.979.

Lack of Toll-like Receptor 4 and 2 Polymorphisms in Korean Patients with Bacteremia

• Yoon HJ
• Choi JY
• Kim CO
• Park YS
• Kim MS
• Kim YK
• Shin SY
• Kim JM
• Song YG

• KMID: 1713104
• DOI: http://doi.org/10.3346/jkms.2006.21.6.979

Abstract

Toll-like receptors (TLRs) are pattern-recognition receptors that are important in innate immune responses to bacterial infection. The purpose of this study is to describe the prevalence of TLRs genetic variations in the bacteremic patients in Korea. A total of 154 patients with bacteremia and 179 healthy volunteers were included. The Asp299Gly and Thr399Ile allele of the TLR4 gene and Arg753Gln and Arg677Trp allele of the TLR2 gene were tested by PCR-RFLP. The DNA sequences were determined to confirm the PCR-RFLP results. Contrary to the expectation, no genetic polymorphisms were detected in both groups of this study, suggesting that it is very rare in Korean.

Keyword

Toll-like Receptors; Polymorphism, Genetic; Bacteremia

MeSH Terms

Toll-Like Receptor 4/blood/*genetics
Toll-Like Receptor 2/blood/*genetics
Risk Factors
Risk Assessment/*methods
Prevalence
Polymorphism, Single Nucleotide/genetics
Middle Aged
Male
Korea/epidemiology
Humans
Genetic Screening/methods
Genetic Predisposition to Disease/epidemiology/genetics
Female
DNA Mutational Analysis
Biological Markers/blood
Bacteremia/blood/*epidemiology/*genetics

Figure

• 

  Fig. 1 Representative results of samples tested for TLR 2 and 4 polymorphisms with the PCR-RFLP. M: DNA 100 bp ladder size marker; 1: TLR4 (Arg299Gly) positive control (223 bp); 2-3: TLR4 (299) in bacteremia (249 bp); 4-5: TLR4 (299) in healthy control (249 bp); 6-7: TLR4 (399) in bacteremia (410 bp); 8-9: TLR4 (399) in healthy control (410 bp); 10-11: TLR2 in bacteremia (3 bands: 38 bp, 75 bp, 227 bp); 12-13: TLR2 in healthy control (3 bands: 38 bp, 75 bp, 227 bp); λ: DNA 50 bp ladder size marker.

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