Immune Netw.  2003 Sep;3(3):176-181. 10.4110/in.2003.3.3.176.

Adhesion of CD40-stimulated Germinal Center B Cells to HK Cells Employs the CD11a/CD18-CD54 Interactions

• Lee J
• Choe J

• KMID: 1615308
• DOI: http://doi.org/10.4110/in.2003.3.3.176

Abstract

BACKGROUND
The molecular basis of follicular dendritic cells (FDC)-germinal center (GC) B cell interaction is largely unknown, although this cellular interaction is thought to be important for the whole process of GC B cell differentiation. METHODS: Using FDC-like cells, HK, and highly purified GC B cells, we attempted to identify the molecules that play critical roles in the interactions between FDC and B cells. GC B cells were co-cultured with HK cells and soluble CD154 in the presence or absence of various function-blocking monoclonal antibodies to examine their effect on GC B cell binding to HK cells and B cell proliferation. RESULTS: Anti-CD11a and anti-CD54 antibodies inhibited GC B cell binding to HK cells while anti-CD49d and anti-CD106 antibodies did not. GC B cell proliferation was not impaired by the disruption of GC B cell-HK cell adherence. CONCLUSION: Our results suggest that CD11a/CD18-CD54 interactions play an important roles in the initial binding of GC B cells to FDC and diffusible growth factors from FDC may be responsible the massive proliferation of GC B cells.

Keyword

Follicular dendritic cell; germinal center B cell; CD40; CD11a/CD18-CD54

MeSH Terms

Antibodies
Antibodies, Monoclonal
B-Lymphocytes*
Cell Communication
Cell Differentiation
Cell Proliferation
Dendritic Cells, Follicular
Germinal Center*
Intercellular Signaling Peptides and Proteins
Antibodies
Antibodies, Monoclonal
Intercellular Signaling Peptides and Proteins