J Korean Soc Ther Radiol Oncol.  2001 Dec;19(4):389-396.

The Differentially Expressed Genes by Radiotherapy in the Patients with Uterine Cervix Cancer

• Seo EY
• Cho MJ
• Lee JH
• Lee YS
• Na MH
• Lee WH
• Kim JS
• Kim JS

Abstract

PURPOSE: To detect differentially expressed genes in the patients with uterine cervical cancer during the radiation therapy.
MATERIALS AND METHODS
In patients with biopsy proven uterine cervical cancer, we took a tumor tissue just before radiation therapy and at 40 minutes after external irradiation of 1.8 Gy. Total RNAs isolated from non-irradiated and irradiated tumor tissue samples were analyzed using the differential-display reverse transcription-polymerase chain reaction (DDRT-PCR). Complementary DNA (cDNA) fragments corresponding to differentially expressed messenger RNAs(mRNAs) were eluted, and cloned. The differential expression of the corresponding mRNAs was confirmed by reverse northern blot. Differentially expressed cDNA bands were sequenced. Nucleotide sequence data were analyzed in the Gene Bank and EMBL databases via the BLAST network server to identify homologies to known genes or cDNA fragments. Expression pattern of down-regulated clone was examined using RT-PCR in 5 patients undergoing radiotherapy.
RESULTS
We identified 18 differentially expressed bands by DDRT-PCR, which were eluted and cloned. There were 10 up-regulated clones and 1 down-regulated clone in reverse northern blot. One cDNA fragment had homology to chemokine receptor CXCR4, four were identified as Human ESTs in the EMBL database in EST clones. Down-regulated CxCa-11 was also down regulated in all patients.
CONCLUSION
Using the DDRT-PCR, we have identified 10 up-regulated and 1 down-regulated clone(s) in the patients with uterine cervix cancer during the radiation therapy. The clinical relevance and the functions of these genes will be further investigated.

MeSH Terms

Base Sequence
Biopsy
Blotting, Northern
Cervix Uteri*
Clone Cells
DNA, Complementary
Expressed Sequence Tags
Female
Humans
Radiotherapy*
RNA
RNA, Messenger
Uterine Cervical Neoplasms
DNA, Complementary
RNA
RNA, Messenger