Int J Oral Biol.
2012 Sep;37(3):109-114.
Identification of differentially expressed genes using an annealing control primer system in periodontitis
- Affiliations
-
- 1Department of Oral Microbiology, School of Dentistry, Pusan National University, Yangsan, Korea. jchung@pusan.ac.kr
Abstract
- In the gingival tissues of patients with periodontitis, inflammatory responses are mediated by a wide variety of genes. In this study, we screened for differentially expressed genes (DEGs) in periodontitis compared with normal tissue using an annealing control primer (ACP) system. By ACP RT-PCR analysis, we obtained about 160 amplicons, 8 of which were found to be differentially expressed. DEGs in patients with periodontitis were thus successfully and reliably identified by the ACP-based RT PCR technique. The DEGs identified in the screen may also enhance our understanding of the pathogenesis of periodontitis.
MeSH Terms
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Identification of Differentially Expressed Genes in Kawasaki Disease Using Annealing Control Primer System
- Search for Differentially Expressed Genes in Preterm Human Placentae and Their Membranes by Annealing Control Primer System
- Identification of CCL1 as a Gene Differentially Expressed in CD4+ T Cells Expressing TIM-3
- Identification of Differentially Expressed Genes in Papillary Thyroid Cancers
- Identification of Differentially Expressed Genes by Gabapentin in Cultured Dorsal Root Ganglion in a Rat Neuropathic Pain Model
