Korean J Perinatol.  2005 Dec;16(4):300-308.

Search for Differentially Expressed Genes in Preterm Human Placentae and Their Membranes by Annealing Control Primer System

• Ko HS
• Kim YH
• Kil KC
• Jeong JH
• Yang DE
• Chung DY
• Park TC
• Kim SP
• Shin JC

Abstract

OBJECTIVE
The aim of this study was to investigate the gene expression profiles using GeneFishingTM kit in human placentae and their membranes delivered at preterm caused by preterm labor.
METHODS
Specimens were obtained from placenta, chorion, and amnion delivered at preterm and term, respectively. Total RNAs were isolated from each specimen. Thereafter, the profiles of expression genes between preterm and term specimens were compared using a new and accurate reverse transcription-polymerase chain reaction (RT-PCR) that involves annealing control primers (ACPs) to identify the genes expressed differentially and screened by basic local alignment search tool (BLAST) search.
RESULTS
Using 20 ACPs, 13 differentially expressed genes (DEGs) were identified and sequenced. 7 of them were expressed up-regulation, while 6 were expressed down-regulation in preterm deliveries. A BLAST searches revealed that 11 were known genes and 2 were unknown genes. Among known genes, up-regulated genes were insulin-like growth factor II associated protein, vigilin, acyl-Coenzyme A dehydrogenase, tissue inhibitor of metalloproteinase 1 (TIMP1), ribosomal protein S26 (RPS26), follistatin-like 1 (FSTL1) and down-regulated genes were two mitochondrial DNAs, ribosomal protein S28 (RPS28), transglutaminase 2 (TGM2), heparin sulfate proteoglycan (HSPG, perlecan).
CONCLUSION
This study shows that the ACP system is a good method for the identification of preterm-related genes. Furthermore, this study suggests that further analysis of the differentially expressed genes in preterm we have identified should provide insights into the molecular basis of preterm delivery caused by preterm labor.

Keyword

Preterm; GeneFishing; Annealing control primers (ACPs)

MeSH Terms

Acyl-CoA Dehydrogenase
Amnion
Chorion
DNA, Mitochondrial
Down-Regulation
Female
Heparin
Humans*
Insulin-Like Growth Factor II
Membranes*
Obstetric Labor, Premature
Placenta*
Pregnancy
Proteoglycans
Ribosomal Proteins
RNA
Tissue Inhibitor of Metalloproteinase-1
Transcriptome
Up-Regulation
Acyl-CoA Dehydrogenase
DNA, Mitochondrial
Heparin
Insulin-Like Growth Factor II
Proteoglycans
RNA
Ribosomal Proteins
Tissue Inhibitor of Metalloproteinase-1