Korean J Clin Microbiol.
2003 Sep;6(2):149-155.
Prevalence & Diversity of Extended-spectrum beta -Lactamase-Producing Escherichia coli and Klebsiella pneumoniae Isolates in Korea
- Affiliations
-
- 1Department of Laboratory Medicine, Colleage of Medicine, Pochon CHA University, Korea.
- 2Department of Laboratory Medicine, Gyeongsang Natational University School of Medicine, Korea.
- 3Department of Laboratory Medicine, Colleage of Medicine Kosin University, Korea.
- 4Department of Laboratory Medicine, Pusan Natatinal University Colleage of Medicine, Korea.
- 5Department of Laboratory Medicine, Sooncheonhyang University Colleage of Medicine, Korea.
- 6Department of Laboratory Medicine, Chonnam Natatinal University Medicine School, Korea.
- 7Department of Laboratory Medicine, Chonbuk University Medicine School, Korea.
- 8Department of Laboratory Medicine, Hanllym University Colleage of Medicine, Korea.
- 9Department of Laboratory Medicine, Yonsei University Wonju Colleage of Medicine, Korea.
- 10Department of Laboratory Medicine, Yonsei University Colleage of Medicine, Korea. leekcp@yumc.yonsei.ac.kr
Abstract
-
BACKGROUND: Increase in extended-spectrum -lactamase(ESBL)-producing Escherichia coli and Klebsiella pneumoniae have been reported in Korea. The aim of this study was to determine the nationwide prevalence of ESBL-producing E. coli and K. pneumoniae, and to investigate the types of ESBLs.
METHODS
A total of 2,221 E. coli and 1,128 K. pneumoniae consecutive isolates were yearly collected from 12 hospitals in 1999 and 2000. ESBL production was performed by National Committee for Clinical Laboratory Standards methods and double synergy tests. The type of ESBL was determined by polymerase chain reaction (PCR), isoelectric focusing, and nucleotide sequence analysis.
RESULTS
ESBL-producing E. coli and K. pneumoniae isolates were detected from all 12 hospitals participated. The proportion of ESBL-producers was 9.1%(2.0-19.6%) of the E. coli and 29.2% (10.0-60.8%) of the K. pneumoniae isolates. Among the 22 isolates sequenced, SHV-12 was found in six isolates, SHV-2a in three isolates, TEM-52 in five isolates, TEM-106 in three isolates, and each of TEM-15, TEM-20, TEM-43, and TEM-107 in one isolate. CTX-M-14 was also found in one isolate.
CONCLUSION
ESBL-producing E. coli and K. pneumoniae are widespred to all levels of Korean hospitals. The most common types of ESBLs in Korea are SHV-12, SHV-2a, and TEM-52. In addition, we also identified new TEM-derived ESBLs.
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- In Vitro Susceptibility of piperacillin/tazobactam Against extended-spectrum beta-lactamase-producing Escherichia coli and Klebsiella pneumoniae
- Evaluation of the Method to Screen Isolates of Extended-Spectrum -Lactamase-Producing Klebsiella pneumoniae and Escherichia coli Using Cefpodoxime Disk
- Antibiogram of Escherichia coli and Klebsiella spp. Detected by Vitek ESBL Test
- Comparison of MicroScan and Phoenix Automated Systems for Detection of Extended-Spectrum beta-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae
- Prevalence of TEM- and SHV-type Beta-lactamase gene in Escherichia coli and Klebsiella pneumoniae in Korea
