J Korean Assoc Oral Maxillofac Surg.  2006 Jun;32(3):200-208.

Expression and functional characterization of amino acid transport system L in Saos2 human osteogenic sarcoma cells

Affiliations
  • 1Department of Oral and Maxillofacial Surgery, College of Dentistry, Chosun University, Korea.
  • 2Department of Oral Physiology, College of Dentistry, Chosun University, Korea. kdk@chosun.ac.kr

Abstract

inhibitor, 2-aminobicyclo-(2,2,1)-heptane-2-carboxylic acid (BCH). The affinity of [14C]L-leucine uptake and the inhibition profiles of [14C]L-leucine uptake by various amino acids in the Saos2 cells were comparable with those for the LAT1 expressed in Xenopus oocytes. The majority of [14C]Lleucine uptake is, therefore, mediated by LAT1 in the Saos2 cells. These results suggest that the transports of neutral amino acids including several essential amino acids into Saos2 human osteogenic sarcoma cells are for the most part mediated by LAT1. Therefore, the Saos2 human osteogenic sarcoma cells are excellent tools for examine the properties of LAT1. Moreover, the specific inhibition of LAT1 in tumor cells might be a new rationale for anti-tumor therapy.

Keyword

Nutrition to tumor cells; L-type amino acid transporters; Saos2 cells; Essential amino acids; Anti-tumor therapy

MeSH Terms

Amino Acid Transport System L*
Amino Acids
Amino Acids, Essential
Amino Acids, Neutral
Humans*
Oocytes
Osteosarcoma*
Xenopus
Amino Acid Transport System L
Amino Acids
Amino Acids, Essential
Amino Acids, Neutral
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