Korean J Anat.  2001 Jun;34(3):253-260.

Immunohistochemical Study on the TfBP Expression in the Embryonic Chick Cerebellum

• Oh SW
• Seo JH
• Park SW
• Kim DW
• Lee C
• Roh EJ
• Jeon GS
• Kang TC
• Lee KH
• Cho SS

Abstract

We have previously demonstrated that transferrin binding protein (TfBP) is a reliable marker for mature oligoden-drocytes (OLGs) in the avian central nervous system (CNS). Unlike mammalian CNS in which OLGs are generated largely postnatally, avian OLGs are differentiated during embryonic development of CNS. In this study, several aspects of TfBP(+/-) OLG development were immunohistochemically examined in the embryonic chick cerebellum : (1) change in shapes of immature cells with respect to time and to location within the cerebellum, (2) possible sites of origin, and (3) pathways of precursor cell migration. Our results indicate that TfBP expression gradually increases and extends from the deep portion of the white matter to gray matter with proportion to progress of cerebellar development. A few TfBP? cells were first observed in the deep portion of the cerebellum at E9. At E13, TfBP(+/-) cells were distributed evenly within the white matter. At E17, many TfBP(+/-) OLGs were located at granular layer and at the near place of Purkinje cell layer. At E20, a large number of TfBP cells appeared at the granular layer with a few in the molecular layer. Our data demonstrated distinct patterns of morphology and location of TfBP(+/-) OLGs in the cerebellum during development and suggest a role of TfBP in OLG development.

Keyword

Transferrin binding protein; Oligodendrocyte; Chick Embryo; Cerebellum

MeSH Terms

Animals
Carrier Proteins
Cell Movement
Central Nervous System
Cerebellum*
Chick Embryo
Embryonic Development
Female
Oligodendroglia
Pregnancy
Transferrin
Carrier Proteins
Transferrin