The relationship of the Coronary Artery Disease and Paraoxonase Gene Polymorphism

You, Kyong Hoon; Kim, Seok Yeon; Kim, Hyo Soo; Sohn, Dae Won; Oh, Byung Hee; Lee, Myoung Mook; Park, Young Bae; Choi, Yun Shik; Lee, Young Woo

Abstract

OBJECTIVES
Paraoxonase is a high density lipoprotein (HDL)-associated enzyme, which has been implicated in preventing low density lipoprotein-cholesterol (LDL-C) from oxidation. The human paraoxonase gene is codominantly expressed as allele A and B. The A allele codes for glutamine (A subtype) and the B allele for arginine (B subtype) at codon 192 of the paraoxonase enzyme. This genetic polymorphism divides the enzyme into high and low activity form. It has been believed that this difference of specific activity might change the metabolism of cholesterol and the prevalence of coronary artery disease. The present study investigated the association among the paraoxonase gene polymorphism and the level of plasma lipoprotein and coronary artery disease.
METHODS
The 416 subjects who have undergone coronary angiography in SNUH were recruited. The patients (n=251) had >50% stenosis of at least one of the major coronary arteries. To identify the genotype of paraoxonase, we amplified the target region in the paraoxonase gene by PCR ( polymerase chain reaction) and electrophoresed the products.
RESULTS
There was no difference between the two groups in the allele frequency (A : B = 0.41 : 0.59 in patients, A : B = 0.37 : 0.63 in controls; p=0.21) or in the genotype frequency (AA:AB:BB= 45:116:90 in patients, AA:AB:BB=22:77:66 in controls; p=0.41). There was no association of the paraoxonase genotype with serum lipoprotein level and acute coronary syndrome in this study. The B allele was not an independent risk factor for coronary artery disease in this study.
CONCLUSION
The paraoxonase gene 192 polymorphism was not an independent risk factor for coronary artery disease in this study.