Korean Circ J.  2003 Feb;33(2):104-112. 10.4070/kcj.2003.33.2.104.

Relationship of Polymorphisms in the Oxidative Stress Related Genes - Paraoxonase and p22phox - to Variant Angina and Coronary Artery Stenosis in Korean

• Cho YS
• Choi JH
• Zhang SY
• Lee JH
• Hwang KK
• Kim HS
• Oh BH
• Lee MM
• Park YB
• Choi YS

• KMID: 2029612
• DOI: http://doi.org/10.4070/kcj.2003.33.2.104

Abstract

BACKGROUND AND OBJECTIVES
Oxidative stress plays an important role in the pathogenesis of coronary atherosclerosis and spasm. We investigated whether the polymorphisms in two oxidative stress-related genes, paraoxonase and p22phox, are associated with risks of coronary artery spasm and stenosis.
SUBJECTS AND METHODS
The study comprised of 116 patients with variant angina, 118 patients with coronary artery stenosis and 117 control subjects, who were all classified by coronary angiography. In all three groups, the genotype frequencies of the Q192R polymorphism of the paraoxonase gene and C242T polymorphism of the p22phox gene were analyzed, and the serum thiobarbituric acid-reactive substance concentrations measured.
RESULTS
The frequency of the RR genotype of the paraoxonase Q192R polymorphism was significantly higher in patients with variant angina and coronary artery stenosis than in the control subjects (40.4% in variant angina and 37.8% in coronary artery stenosis vs. 24.7% in control, p=0.020 and 0.048, respectively). From the multivariate analysis, the odds ratio of the RR genotype was 2.240 for variant angina (95% confidence interval ; 1.012-4.956), and 2.333 for coronary artery stenosis (95% confidence interval ; 1.140-4.777), in relation to the control subjects. The thiobarbituric acid-reactive substance level was significantly higher in the RR type than in the QQ+QR types (RR vs. QQ+QR : 1.106+/-0.420 nmol/mL vs. 0.949+/-0.311 nmol/mL, p=0.028). There was no significant difference in the prevalence of the C242T polymorphism of the p22phox gene between the three groups.
CONCLUSION
The RR genotype of the paraoxonase gene Q192R polymorphism was found to be an independent risk factor for both coronary spasm and stenosis.

Keyword

Oxidative stress; Angina pectoris; Coronary stenosis; Polymorphism, single nucleotide

MeSH Terms

Angina Pectoris
Aryldialkylphosphatase*
Constriction, Pathologic
Coronary Angiography
Coronary Artery Disease
Coronary Stenosis*
Coronary Vessels*
Genotype
Humans
Multivariate Analysis
Odds Ratio
Oxidative Stress*
Polymorphism, Single Nucleotide
Prevalence
Risk Factors
Spasm
Aryldialkylphosphatase