J Korean Acad Oral Maxillofac Radiol.  1998 Feb;28(1):171-191.

The Effects Of Calcium-Deficient Diet After Irradiation On The Periodontal Tissue Formation In Rat Pups

• Jun CD
• Hwang EH
• Lee SR

Abstract

The present study was deigned to elucidate the effects of the Co-60 gamma irradiation and/or calcium-deficient diet on the periodental tissue formation in rat pups. The pregnant three-week old Sprague-Dawley rats were used for the study. The experimental groups was divided into two groups, irradiation/normal diet group(Group 2) and irradiation/calcium-dificient diet group(Group 1). The abdoment of the rats at the 19th day of pregnancy were irradiated with single absorbed dose of 350 cGy. The rats pups were sacrificed on the 14th day after delivery, and the maxillae including molar tooth sections for light and trasmission electron microscopy. Some of tissue sections for light microscopy were stained immunohistochemically with anti-fibronectin antibodies. The results were as follows ; 1. In the periodontal ligament forming area, the fibroblasts of Group 2 showed irregular arrangement and low activity. The immunoreactivity between the fibroblasts and collagen fibers was decreased, compared with Group 1. The fibroblasts of Group 3 showed atrophic change and clumped nucleus. The collagen fibers showed cystic change and low immunoreactivity to the fibronetin. 2. In the cementum forming area, the cementoblasts of Group 2 showed decrease of number and atrophic change. The cementoblasts of Group 3 showed edematous change, atrophy of cytoplasm, and clumping of nucleus. 3. In the alveolar bone forming area, the bone of Group 2 was thin and various degree of immunoreactivity to the osteonectin, Group 3 showed edematous osteoblasts, fibrous degeneration of bone marrow, and weak immunoreactivity tothe osteonectin.

MeSH Terms

Animals
Antibodies
Atrophy
Bone Marrow
Collagen
Cytoplasm
Dental Cementum
Diet*
Fibroblasts
Maxilla
Microscopy
Microscopy, Electron
Molar
Osteoblasts
Osteonectin
Periodontal Ligament
Pregnancy
Rats*
Rats, Sprague-Dawley
Tooth
Antibodies
Collagen
Osteonectin