J Korean Acad Oral Maxillofac Radiol.
1998 Feb;28(1):87-110.
The Effect Of Irradiation And Calcium-Deficient Diet On Dentin And Cementum Formation Of Rat Molar
- Affiliations
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- 1Department of Oral & Maxillofacial Radiology, College of Dentistry, Kyung Hee University, Korea.
Abstract
- The present study was designed to elucidate the effects of the Co-60 r irradiation and/or calcium deficient diet on the dentin and cementum formation of rat molar. The pregnant three-week old Sprague-Dawley rat were used for the study. The experimental group was divided into two groups, irradiation/normal diet group and irradiation/calcium-deficient diet group. The control group was non-irradiation/normal diet group. The abdomen of the rats at the 19th day of pregnancy were irradiated with single absorbed dose of 350 cGy. The rat pups were sacrificed on the 14th day after delivery and the maxillae including molar tooth germ were taken. The specimens including the 1st molar tooth germ were prepared to make tissue sections for light and trasmission electron microscopy. Some of tissue sections for light microscopy were stained immunohistochemically with anti-fibronectin antibody. The results were as follows ; 1. The Hertwig s epithelial root sheath cells, which are related to the differentiation of the tooth-forming cells, showed irregular cellular arrangement, decrease of intercellular junctional complex, and decreased immunoreactivity to the fibronectin after irradiation. These were more severe in the irradiation/calcium-deficient diet group. 2. The cementoblasts at the cementum-forming area showed chormatin clupings after irradiation. The immu noreactivity to the fibronectin was weaken after irradiation, especially irradiation/calcium-deficient diet group. 3. The odontoblast at the dentin-forming area showed increase of lysosomes in the cytoplasm and destruction of intercellular junctional complex. The irradiation/calcium-deficient diet group showed decrease of number and density of th electron dense particles and a large number of vacuoles scattered in the dentin matrix. The immunoreactivity was weaken.
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