Exp Mol Med.
1997 Dec;29(4):235-237.
A simple protocol of DNA sequencing with 10% formamide for dissolving G/C compression
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- 1GYEONGSANG NATL UNIV, COLL MED, DEPT BIOCHEM, CHINJU 660280, SOUTH KOREA.
Abstract
- Formamide has been widely used in urea/polyacrylamide gel to solve the compression problems that are occasionally found during the DNA sequencing of G/C rich regions. In this study, however, 10% formamide was added in annealing solution in stead of adding to the gel. The compressions were unfolded efficiently with a more rapid annealing reaction on ice in the presence of 10% formamide.
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