J Dent Rehabil Appl Sci.  2017 Jun;33(2):106-113. 10.14368/jdras.2017.33.2.106.

Interaction of Hydroxyethylidene bisphosphonate (HEBP) with other endodontic irrigants on tissue dissolving capacity and antimicrobial effect

• Kim R
• Kim YG
• Kim MY
• Song BC
• Kim SH
• Kim JH

• KMID: 2388016
• DOI: http://doi.org/10.14368/jdras.2017.33.2.106

Abstract

PURPOSE
The purpose of this study was to evaluate tissue dissolving capacity, antimicrobial effect of Hydroxyethylidene bisphosphonate (HEBP) interacting with sodium hypochlorite (NaOCl), Ethylenediaminetetraacetic acid (EDTA) as conventional endodontic irrigants and to determine tissue dissolving efficacy depended on temperature.
MATERIALS AND METHODS
A total of 80 bovine muscles were randomly distributed into 8 groups (n = 10). After their initial weights determined on a precision scale, the specimens in each group were immersed in the solutions for 5, 10 and 15 min and reweighted at each time period. Agar diffusion test inoculated with Enterococcus faecalis was performed for antimicrobial effect of each endodontic irrigants.
RESULTS
The ability to dissolve organic matter was greater in NaOCl group following NaOCl and HEBP mixture. Heated NaOCl (40℃) and NaOCl/HEBP mixture was greater tissue dissolving efficacy than room temperature (25℃). Antimicrobial effect was greater and significant in the following order EDTA > EDTA + 1% NaOCl > 1% NaOCl ≥ 1% NaOCl + HEBP.
CONCLUSION
HEBP as soft chelating agent does not disturb antimicrobial effect and less affected tissue dissolving efficacy as inherent properties of NaOCl. In the heated NaOCl/HEBP mixture analyzed, it dissolved more the organic matter than room temperature.

Keyword

HEBP (Hydroxyethylidene bisphosphonate); endodontic irrigants; soft chelating agent; tissue dissolving capacity; antimicrobial effect

MeSH Terms

Agar
Diffusion
Edetic Acid
Enterococcus faecalis
Hot Temperature
Muscles
Sodium Hypochlorite
Weights and Measures
Agar
Edetic Acid
Sodium Hypochlorite

Figure

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  Fig. 1 (A) General Lan Incubator IB 05-G (60L, Jeio Tech, Inc., Seoul, Korea), (B) Model for Petri dish set up.

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  Fig. 2 The mean rate of weight changes (%) after 5 min, 10 min and 15 min of submersion to the irrigation solutions. EDTA, etylenediaminetetraacetic acid; HEBP, hydroxyethylidene bisphosphonate; NaOCl, sodium hypochlorite.

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  Fig. 3 The effect of HEBP. These graphs show comparisons of the mean rate of weight change (%) of Group 4, 6 and 7 after 10 min and 15 min submersion to the irrigation solutions. Values are shown as the mean. NaOCl, sodium hypochlorite; EDTA, etylenediaminetetraacetic acid; HEBP, hydroxyethylidene bisphosphonate.

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  Fig. 4 The effect of temperature increase. These graphs show comparisons of the mean rate of weight change (%) of 25°C and 40°C groups after 10 min and 15 min of submersion to G4 and G5 (A), G7 and G8 (B). Values are shown as the mean. NaOCl, sodium hypochlorite; HEBP, hydroxyethylidene bisphosphonate.

Reference

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