Korean J Fertil Steril.  1997 Aug;24(2):261-265.

In Vitro Development of Mouse IVF/IVC Embryo Treated with Epidermal Growth Factor (EGF)

• Kim EY
• Kim MK
• Yi BK
• Lee HS
• Yoon SH
• Park SP
• Chung KS
• Lim JH

Abstract

SUMMARY: The objective of this study was to examine the effect of EGF to the in vivo development of mouse IVF/IVC embryo. The 2-cell embryos were cultured in medium (5-6 embryos/25 microliter/drop) w/w0 EGF (10 ng/ml) and day 4 blastocysts recovered from each treatment group were transferred into the uteri of recipients of pseudopregnant day 3. The results obtained in this experiment were summarized as follows: 1. When the effect of EGF to the in vitro development and cell number of blastocysts produced from the culture of 2-cell embryos in w/w0 EGF was determined, those results of EGF treatment group showed not significant difference compared with control. 2. However, when the effect of EGF to the in vivo development of blastocysts recovered from each treatment group was examined, production of the normal fetus against transferred embryos in EGF treatment group (51.2%) was very higher than that in control group (31.1%), although total implantation was not significantly different between treatment group (control: 64.4%, EGF: 69.8%). Therefore, this result suggested that EGF can affect to the in vivo development of IVF/IVC embryos through the improvement of embryo quality, although EGF treated embryos showed not significant development rate compared with control.

MeSH Terms

Animals
Blastocyst
Cell Count
Embryonic Structures*
Epidermal Growth Factor*
Fetus
Mice*
Uterus
Epidermal Growth Factor