J Korean Surg Soc.  2007 Aug;73(2):138-145.

Expression of Panendothelial and Lymphatic Vessel Markers of a Pre-operative Biopsy Specimen from Colorectal Cancer

• Han GS
• Kim BG
• Cha SJ
• Chang IT
• Lee TJ

Abstract

PURPOSE: Panendothelial markers such as factor VIII, CD34, CD31, CD105 (endoglin) and D2-40 are useful to identify proliferating endothelium that is related to tumor invasion. This study was designed to identify the correlation between the expressions of panendothelial and lymphatic vessel markers in preoperative biopsy specimens and the clinicopathologic factors.
METHODS
Preoperative biopsy specimens from 72 patients were immunostained for CD105, CD34, CD31, Factor VIII and D2-40. The microvessel and lympathic vessel densities (MVD and LVD) were counted in dense vascular foci (hot spots) on a x200 field in each specimen. The correlation between these factors and the clinicopathologic parameters were analyzed.
RESULTS
The MVD by CD105 showed statistically significant correlation with tumor emboli, the T-stage, nodal metastasis and the stage, and the MVD by CD34 had statistically significant correlation with tumor emboli, nodal metastasis and the stage. The lympathic vessel density (LVD) by D2-40 showed a statistically significant correlation with tumor emboli, the T-stage and nodal metastasis.
CONCLUSION
The MVD by CD105 and the LVD by D2-40 in preoperative biopsy specimens of colorectal cancers may be useful markers for the prediction of invasiveness.

Keyword

Colorectal cancer; CD105; CD34; CD31; Factor VIII; D2-40

MeSH Terms

Biopsy*
Colorectal Neoplasms*
Endothelium
Factor VIII
Humans
Lymphatic Vessels*
Microvessels
Neoplasm Metastasis
Factor VIII