Immunohistochemical study of odontogenic keratocyst

Lee, Sang Gu; Lee, Jae Hoon; Kim, Chul Hwan

Abstract

The odontogenic keratocyst(OKC) represents approximately 11% of odontogenic cysts and is decided by microscopic and histopathologic determinant rather than by clinical appearance. The OKC is noted for its capacity to recur after treatment. In this study, the immunohistochemical expression of recurrent OKCs and non-recurrent OKCs after cyst enucleation were examined. The PCNA, Ki-67 and cytokeratin (low molecular weight and high molecular weight) antibody staining were used. The obtained results were as follows. In proliferating rate of Non-recurrent OKCs and recurrent OKCs using PCNA and Ki-67 staining, There was not significant difference. The positive reaction rates of PCNA and Ki-67 staining were higher in inflamed case than in non-inflamed case. In cytokeratin (low molecular weight) staining, unspecific pattern and low-grade positive reaction were showed both non-recurrent OKCs and recurrent OKCs. Tn cytokeratin(high molecular weight) staining of non-recurrent OKCs, the unspecific pattern and low-grade positive reaction has shown. But, in recurrent OKCs, the strong positive reaction in basal third of epithelium has shown. From the above results, it was suggested that the recurrence of OKCs was not related to proliferating rate, which is influenced by inflammation or surrounding environments and differential rate of basal third influenced recurrent rate.