Korean J Oral Maxillofac Radiol.  2004 Sep;34(3):137-144.

Effects of low dose irradiation on the calcific nodule formation in MC3T3-E1 osteoblastic cell line

• Kim KA
• Koh KJ

Abstract

PURPOSE
To investigate the effects of low dose irradiation on the calcium content and calcific nodule formation of the MC3T3-E1 osteoblastic cell line. MATERIALS AND METHODS: Cells were irradiated with a single dose of 0.2, 0.4 and 0.6 Gy at a dose rate of 5.38 Gy/min using Cs-137 irradiator. After irradiation, the calcium content and calcific nodule formation were examined on the 1st, 2nd, 3rd and 4th week. RESULTS: We did not find any significant difference of total calcium content after irradiation of 0.2, 0.4 and 0.6 Gy when compared with the unirradiated control group. There was no significant difference of total calcium content between 0.2, 0.4 and 0.6 Gy irradiated groups. We found an increased tendency of the calcific nodule formation after irradiation of 0.2, 0.4 and 0.6 Gy when compared with the unirradiated control group without significant difference of calcific nodule formation between 0.2, 0.4 and 0.6 Gy irradiated groups. CONCLUSION: The results showed an increased tendency of the calcific nodule formation after low dose irradiation. However, this tendency did not increase with the increase of irradiation dose.

Keyword

Radiation; Low Dose; Osteoblastic Cell Line; Calcification

MeSH Terms

Calcium
Cell Line*
Osteoblasts*
Calcium