Korean J Oral Maxillofac Radiol.  2008 Sep;38(3):125-132.

Effects of irradiation on TGF-beta1 mRNA expression and calcific nodule formation in MC3T3-E1 osteoblastic cell line

• Song JS
• Kim KA
• Koh KJ

Abstract

PURPOSE: To investigate the effects of irradiation on transforming growth factor beta1 (TGF-beta1) mRNA expression and calcific nodule formation in MC3T3-E1 osteoblastic cell line.
MATERIALS AND METHODS
Cells were cultured in alpha-minimum essential medium (alpha-MEM) supplemented with 10% fetal bovine serum and antibiotics. When the cells reached the level of 70-80% confluence, culture media were changed with alpha-MEM supplemented with 10% FBS, 5 mM beta-glycerol phosphate, and 50 microgram/mL ascorbic acid. Thereafter the cells were irradiated with a single dose of 2, 4, 6, 8 Gy at a dose rate of 1.5 Gy/min. The expression pattern of TGF-beta1 mRNA, calcium content and calcific nodule formation were examined on day 3, 7, 14, 21, 28, respectively, after the irradiation.
RESULTS
The amount of TGF-beta1 mRNA expression decreased significantly on day 7 after irradiation of 4, 6, 8 Gy. It also decreased on day 14 after irradiation of 6, 8 Gy, and decreased on day 21 after irradiation of 8 Gy. The amount of calcium deposition decreased significantly on day 7 after irradiation of 4, 8 Gy (P.0.01) and showed a decreased tendency on day 14, 21 after irradiation of 4, 6, 8 Gy. The number of calcific nodules was decreased on day 7 after irradiation of 4, 8 Gy.
CONCLUSION
Irradiation with a single dose of 4, 6, 8 Gy influences negatively the bone formation at the molecular level by affecting the TGF-beta1 mRNA expression that was associated with proliferation and the production of extracellular matrix in MC3T3-E1 osteoblastic cell line.

Keyword

TGF-beta1; calcific nodule; MC3T3-E1 osteoblastic cell line; Irradiation

MeSH Terms

Anti-Bacterial Agents
Ascorbic Acid
Calcium
Cell Line
Culture Media
Extracellular Matrix
Glycerophosphates
Organic Chemicals
Osteoblasts
Osteogenesis
RNA, Messenger
Transforming Growth Factor beta1
Anti-Bacterial Agents
Ascorbic Acid
Calcium
Culture Media
Glycerophosphates
Organic Chemicals
RNA, Messenger
Transforming Growth Factor beta1