Nucl Med Mol Imaging.  2007 Feb;41(1):42-48.

Imaging of Lung Metastasis Tumor Mouse Model using [18F]FDG Small Animal PET and CT

Affiliations
  • 1Laboratory of Nuclear Medicine, Korea Institute of Radiological and Medical Sciences (KIRAMS), Seoul, Korea. larry@kcch.re.kr
  • 2Department of Nuclear Medicine, Korea Institute of Radiological and Medical Sciences (KIRAMS), Seoul, Korea.

Abstract

PURPOSE: The purpose of this study is to image metastaic lung melanoma model with optimal pre-conditions for animal handling by using [18F]FDG small animal PET and clinical CT.
MATERIALS AND METHODS
The pre-conditions for lung region tumor imaging were 16-22 h fasting and warming temperature at 30 degrees C. Small animal PET image was obtained at 60 min postinjection of 7.4 MBq [18F]FDG and compared pattern of [18F]FDG uptake and glucose standard uptake value (SUVG) of lung region between Ketamine/Xylazine (Ke/Xy) and Isoflurane (Iso) anesthetized group in normal mice. Metastasis tumor mouse model to lung was established by intravenous injection of B16-F10 cells in C57BL/6 mice. In lung metastasis tumor model, [18F]FDG image was obtained and fused with anatomical clinical CT image.
RESULTS
Average blood glucose concentration in normal mice were 128.0+/-23.87 and 86.0+/-21.65 mg/dL in Ke/Xy group and Iso group, respectively. Ke/Xy group showed 1.5 fold higher blood glucose concentration than Iso group. Lung to Background ratio (L/B) in SUVG image was 8.6+/-0.48 and 12.1+/-0.63 in Ke/Xy group and Iso group, respectively. In tumor detection in lung region, [18F]FDG image of Iso group was better than that of Ke/Xy group, because of high L/B ratio. Metastatic tumor location in [18F]FDG small animal PET image was confirmed by fusion image using clinical CT.
CONCLUSION
Tumor imaging in small animal lung region with [18F]FDG small animal PET should be considered pre-conditions which fasting, warming and an anesthesia during [18F]FDG uptake. Fused imaging with small animal PET and CT image could be useful for the detection of metastatic tumor in lung region.

Keyword

melanoma; lung metastastic tumor; [18F]FDG; small animal PET; clinical CT

MeSH Terms

Anesthesia
Animals
Animals*
Blood Glucose
Fasting
Glucose
Injections, Intravenous
Isoflurane
Lung*
Melanoma
Mice*
Neoplasm Metastasis*
Blood Glucose
Glucose
Isoflurane
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